摘要
目的探讨联合免疫磁珠分选和多重探针连接依赖性扩增(MLPA)技术检测多发性骨髓瘤(MM)分子遗传学异常的可靠性。方法收集29例初诊MM患者的骨髓细胞,用CD138磁珠进行分选,设计MLPA探针检测分选前后标本TP53和RB1的表达情况,并与FISH检测结果进行对照。结果 MLPA检测成功率100%,与FISH结果吻合度达99.1%,分选后MLPA和FISH阳性率均有显著性提高。结论 MLPA适合临床MM分子遗传学异常检测,标本应在检测前进行磁珠分选。
Objective To investigate the reliability of magnetic-activated cell sorting （MACS） combined with multiplex ligation-dependent probe amplification （MLPA） in the detection of the molecular cytogenetic abormalities in multiple myeloma. Methods The bone marrow ceils were colelcted from 29 patients with multiple myeloma. The irnmunomagnetically sorted and unsorted ceils were detected for TP53 and RB1 expressions using MLPA probes and the results were compared with fluorescent in situ hybridization （FISH）. Results The detection success rate was 100% for MLPA, which yielded results with an concordance rate of 99.1% with the FISH results. The positivity rates of MLPA and FISH were both increased after immunomagnetic sorting of the bone marrow cells. Conclusion MLPA can well suit the clinical needs for detecting molecular cytogenetic abnormalities in multiple myeloma, and the samples should be immunomagnetically sorted before the assay.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第9期1332-1335,共4页
Journal of Southern Medical University
基金
广东省科技计划项目(2010B031900037)
关键词
多发性骨髓瘤
多重连接依赖性扩增
荧光原位杂交
免疫磁珠分选
multiple myeloma
multiplex ligation dependent probe amplification
fluorescene in situ hybridization
immunomagnetic sorting
