摘要
以量热式农残生物传感器为研究对象,借鉴免疫分析方法,进行竞争性抑制酶联反应在量热式农残生物传感器中的试验研究。结果表明,在甲胺磷浓度为0~0.004 mg.kg-1范围内,甲胺磷含量和放热量之间的线性较好,竞争性抑制酶联反应分析法测农药残留的最低检测限为0.0011 mg.kg-1,每次测定时间为20 min。
Drawing on immunoassay methods,the preliminary test of thermal biosensor was researched in the method of competitive inhibition of enzyme reactions.The results showed that,at concentration of 0-0.004 mg.kg-1 of methamidophos,there is good linearity between methamidophos content and heat release.The limit of detection of competitive inhibition of enzyme reactions method is 0.0011 mg.kg-1,and the measurement time is about 20 minutes.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2012年第8期25-29,共5页
Journal of Northeast Agricultural University
基金
国家自然基金(31101283)
上海市科委重点项目(10391901500)
上海市科技兴农重点攻关项目(2006第7-4号)
关键词
酶标农药
鸡肝酯酶
量热式酶传感器
竞争性抑制酶联反应
enzyme-labeled pesticides
liver esterase
thermal biosensor
competitive inhibition of enzyme reactions
