摘要
目的:2011年5月25日-5月27日,我市苍南县暴发了57例因食用贻贝引起的腹泻性贝类毒素(DSP)中毒事件,采集了19份样品进行检测。方法:采用超高效液相色谱-串联质谱方法对贻贝中5种脂溶性贝类毒素进行检测,根据欧盟贝类毒素的限量值进行评价。结果:15份样品检出大田软海绵酸(OA)、鳍藻毒素-1(DTX-1)和7-epi-pectenotoxin-2 seco acid(7-epi-PTX-2sa),16份检出pectenotoxin-2seco acid(PTX-2sa),7份检出pectenotoxin-2(PTX-2)。其中4份样品中游离OA和DTX-1含量超出欧盟限量值3倍多,它们的总OA约超出9倍。其余均低于欧盟限量值。首次在我国贝类中检出了PTX-2sa和7-epi-PTX-2sa。结论:鉴于部分贻贝样品DSP超标,今后应加强贝类上市前的监测工作。
Objective：During 25 -27th May 2011, there was an outbreak of diarrhetic shellfish poisoning （DSP） after ingestion of intoxicated mussels in Cangnan county, located at the south coast of Zhejiang province in China, 57 persons were affected. To investigate the contamination of shellfish poisoning in mussels based on 19 samples randomly collected from the local seafood markets. Methods： Ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry was used to detect the following lipophilic toxins： okadaic acid （OA）, dinophysistoxin - 1 （ DTX - 1 ）, pectenotoxin - 2 （ PTX - 2）, pectenotoxin - 2 seco acid （ PTX - 2sa） and 7 - epi - pecteno- toxin - 2 seco acid （7 - epi - PTX -2sa）. The safety assessment of diarrhetic shellfish poisons and pectenotoxins in mussels was conducted according to the maximum permitted levels in EU. Results ： OA, DTX - 1 and 7 - epi - PFX -2sa were detected in 15 samples, PTX -2sa was detected in 16 samples, and prFx --2 was detected in 7 samples, respectively. The concentrations of free OA and DTX - 1 in 4 samples were about 3 times greater than the maximum permitted levels in EU, and their total OA surpassed 9 times the level. Others were below the EU regulatory level. This paper for first time reported the detection of PTX - 2sa and 7 - epi - PTX - 2sa in China shellfish. Conclusion： Consequent measures should be taken by regulatory authorities to ensure safety of harvested shellfish product.
出处
《中国卫生检验杂志》
北大核心
2012年第8期1905-1907,1911,共4页
Chinese Journal of Health Laboratory Technology
基金
温州市医学重点学科
温州市第三轮"311"工程建设项目基金(2008012)
