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人芽囊原虫的光学和超微结构研究 被引量:22

Studies of Light Microscope Appearance and Ultrastructure of Blastocystis hominis in Vitro Culture
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摘要 首次在中国记载了人芽囊原虫。利用光学和电子显微镜观察其生活史各阶段的形态结构,显示此虫除前人描述的三种类型之外,还存在一种我们称之为复分裂型 multiple fis-sion form,该型虫体细胞可分裂成3~10余个数量不等的虫体。颗粒型虫体的繁殖颗粒,能在细胞质中产生;阿米巴型虫体形态变化大而活动性较强,但在变形过程中未见虫体移动;空泡型的超微结构显示具有双层膜的细胞核、内质网、核糖体和具双层膜的线粒体,线粒体内的电子半透明的管状或泡状结构,实质上是线粒体的嵴;颗粒型虫体内有4个电子密度均匀的球形颗粒,功能不详。颗粒型虫体也能以二分裂方式繁殖。 The present paper is the first report of a parasitic protozoa Blastocystis ho- minis in China,It deals with its microscope morphology and ultrastructures,basi- ng on living and stained materials isolated from the fecal samples of 3 diarrhea patients and kept apartly in continuons cultures in vitro fo study. Apart trom the 3 forms already reported for the parasite by previous invest- igators,namely,vacuolated form,granular form and ameda form,another form, which we call“multiple fission form”that eventually produces 3 to many new individuals,is here described.Reproductive granules of the granular form were seen to be formed in the cytoplasm of the parasite,Active extension and retrac- tion of pseudopods of the ameba form were seen occasionally,but such activity did not result in cell progression.EM study of the vacuolated form revealed the nucleus with double membranes,rough endoplasmic reticulum,ribosomes and mit- ochondria of double membranes with abundant translucent tubular structures,the cristae.Granular form containing 4 large round granules of unknown function was observed,Ultrastructures of the multiple fission form were also studied.
出处 《中山大学学报(自然科学版)》 CAS CSCD 1990年第3期122-128,共7页 Acta Scientiarum Naturalium Universitatis Sunyatseni
关键词 阿米巴 人芽囊原虫 寄生原虫 Protozoa Amoebida vitro culture diarrhea Blastocystis hominis vacuolated form granular form ameba form multiple fission form
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