摘要
为筛选有效防治烟粉虱的药剂,采用琼脂保湿浸叶法,比较了烟粉虱成虫对生产上广泛喷施的5种药剂5%锐劲特、2.5%高效氯氟氰菊酯、4%阿维菌素、70%艾美乐、25%噻嗪酮的敏感性。对比得出烟粉虱对4%阿维菌素的敏感性较好,药剂处理72 h后成虫校正死亡率高达98.01%。70%艾美乐次之,烟粉虱成虫校正死亡率达80.91%。2.5%高效氯氟氰菊酯较差,处理72 h后成虫死亡率40.73%。25%噻嗪酮最差,处理烟粉虱成虫72 h后死亡率低于5.00%。CYP6CM1、Coe1基因分别是烟粉虱对吡虫啉类药剂、有机磷类药剂产生抗药性的重要响应基因,应用Semi-quantitive PCR检测药剂处理72 h烟粉虱成虫CYP6CM1和Coe1基因转录水平表达量变化。发现70%艾美乐处理后,CYP6CM1基因表达量显著升高,Coe1基因表达量与药剂处理前无明显差异。4%阿维菌素处理后,CYP6CM1基因表达量显著下降,Coe1基因表达量与药剂处理前无明显差异。2.5%高效氯氟氰菊酯处理后CYP6CM1基因表达量变化不大,Coe1基因表达量稍有增加。25%噻嗪酮处理后CYP6CM1基因表达量稍有增加,Coe1基因表达量略有下降。不同药剂对烟粉虱的防治效果与烟粉虱解毒酶基因(CYP6CM1、Coe1)的表达响应有一定的关系。
The whitefly Bemisia tabaci （Gennadius） is one of the most devastating pest insects, and has caused heavy loss of crop yield. B. tabaci has a wide range of host and reproduces fast. The control B. tabaci mainly depends on pesticides. In order to get more effective pesticides, pesticides such as 5% regent, 2.5% lambda-cyhalothrin, 4% abam-ectin, 70% confidor, and 25% buprofezin were investigated for the efficacy by leaf-dip bioassay. After 72- h application, 4% abamectin showed the highest efficacy against B. tabaci, the adjusted mortality of B. tabaci be- ing 98.01%. 70% confidor followed, the adjusted mortal- ity being 80. 91%. B. tabaci exhibited the weakest sus- ceptability to 2. 5% lambda-cyhalothrin with the mortality less than 5.00%. CYP6CM1 and Coel were related toB. tabaci adult resistances to imidacloprid and organophosphates respectively. After B. tabaci adult was deposed to 4% ab- amectin for 72 h, CYP6M1 expression had a significa.nt decrease, and Coel stayed stable. After B. tabaci adult was de- posed to 2.5% lambda-cyhalothrin for 72 h, CYP6M1 expression showed change, while Coe! increased slightly. After B. tabaci adult was deposed to 25% buprofezin for 72 h, CYP6M1 increased slightly, and Coel decreased slightly. It indi- cated that the control of different insecticides on B. tabaci had some negative correlations with transcript expressions of CYP6CM1 and Coel.
出处
《江苏农业学报》
CSCD
北大核心
2012年第3期482-486,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(30971830)
江苏省自然科学基金项目(BK2011676)
江苏省科技支撑计划项目(BE2011309)
江苏省自主创新基金项目[CX(10)101]
关键词
烟粉虱
药剂防治
抗药基因
Bemisia tabaci
chemical control
insecticide resistant gene
