摘要
目的探讨大鼠实验性胃溃疡自愈期间下颌下腺三叶因子1(TFF1)的表达变化。方法采用免疫组织化学和RT-PCR法,分别从蛋白水平及基因水平检测42只溃疡组、21只盐水组和6只正常组大鼠下颌下腺TFF1的表达。结果免疫组织化学SP法显示,正常大鼠下颌下腺颗粒曲管的多颗粒细胞呈TFF1免疫反应阳性,各级导管管腔内也有TFF1阳性物质,纹状管管腔游离面可见线条状TFF1阳性物。溃疡组下颌下腺TFF1肽积分吸光度值明显增加,高于相应的盐水对照组和正常组(P<0.05或P<0.01),其中1d、2d、4d、6d TFF1肽积分吸光度值逐渐增加,6d最高,10d、14d、23d也显著高于盐水对照组。RT-PCR结果显示,下颌下腺有TFF1 mRNA转录,且溃疡组TFF1/GAPDH mRNA吸光度比值在溃疡2～23d均高于盐水对照组和正常组(P<0.01或P<0.05)。结论下颌下腺TFF1肽在大鼠实验性胃溃疡形成和愈合过程中高表达,主要通过导管系统排泄,参与实验性胃溃疡的愈合。
Objective To explore the expression of trefoil factorl （TFFI） gene in submandibular glands during the self-healing of experimental gastric ulcer in rats. Methods Immunohistochemical SP and RT-PCR methods were used to detect the expression of TFF1 peptide and TFF1 mRNA in submandibular glands on 42 gastric ulcer（GU） ,21 normal saline control（NS） and 6 normal （N） rats. Results TFF1 immunoreactive profiles were mainly located in cytoplasma of granular convoluted tubule （GCT） epithelia cells, especially in many granules cell. Substantial amounts of TFF1 were also found in lumen or luminal membrane of striated duct （SD） and interlobular duct （ILD）. In the GUs, especially at the 6-day GU, TFF1 in the lumens of SD and ILD increased. The integral absorbance （IA） values of TFF1 increased obviously in gastric ulcer which was stronger than in the other two groups （ P 〈 0. 05 or P 〈 0.01 ）. On day 1,2,4, 6 the IA values of TFFI peptide increased gradually and kept at the highest level on day 6, then a trend of decrease on day 10-23 ,but still obviously higher than the control group. The optical density of TFF1/GAPDH mRNA on the gastric ulcer group was obviously higher than that of the saline and control group during day 2 to day 23 after gastric ulcer operation（P 〈 0.05 or P 〈 0. 01 ）. Conclusion The TFFI plays a promotive function during the self healing of experimental gastric ulcer by the high expression of TFF1 mRNA /peptide andexcreting into duct system.
出处
《解剖学报》
CAS
CSCD
北大核心
2012年第4期535-539,共5页
Acta Anatomica Sinica
基金
河北省自然科学基金资助项目(C2007000906)
