摘要
[目的]建立一种快速有效的重金属离子毒性检测方法。[方法]选择三角褐指藻(Phaeodactylum tricornutum)为受试生物,采用双通道脉冲振幅调制叶绿素荧光仪(简称ToxY-PAM)检测水中Cu2+、Zn2+、Cr6+3种重金属离子的毒性大小。[结果]三者不同浓度引起的三角褐指藻光合作用抑制率变化基本均在1 h内达到最大值并趋于平稳;3种重金属离子的毒性大小为Cu2+>Zn2+>Cr6+。[结论]ToxY-PAM体积小,携带方便,检测速度快,灵敏度高,适合于重金属离子的现场检测。
[Objective] The research aimed to establish a fast and effective methods about the detection of heavy metal ions toxicity.[Method]Choosing Phaeodactylum tricornutum as testing organisms,and using Dual-channel PAM Chlorophyll Fluorometer to detect Cu^2＋、Zn^2＋、Cr^6＋ in the water.[Result]The changes of photosynthesis inhibition rate caused by different concentrations of the three heavy metal ions almost reached maximum and stabilized in one hour;The order of toxicity intensity among the three heavy metal ions was as below： Cu^2＋Zn^2＋Cr^6＋.[Conclusion] ToxY-PAM instrument has small size and high sensitivity,convenient to carry,and the speed of detection is quickly,so it＇s suitable for detection of heavy metal ion in the field.
出处
《安徽农业科学》
CAS
2012年第22期11375-11376,11413,共3页
Journal of Anhui Agricultural Sciences
关键词
重金属离子
三角褐指藻
叶绿素荧光仪
生物毒性
Heavy metal ion
Phaeodactylum tricornutum
Chlorophyll fluorescence
Biotoxicity
