运动对成长期骨骼肌神经营养因子水平的影响被引量：2

Effects of Activities on Expression of Neurotrophins in Skeletal Muscules in Growth Phase

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摘要目的:肌源性神经营养因子(neurotrophins,NTs)对神经肌肉接头(neuromuscular junc-tion,NMJ)的正常发育起重要作用,旨在分析NTs在成长期(离乳-性成熟)骨骼肌的蛋白水平特征,了解其在NMJ发育中的作用及运动干预的影响。方法:对3周龄SD雄性大鼠分别进行运动增强(跑台运动)和运动减弱(尾部悬吊)干预,测定并比较正常发育及运动干预下几种NTs蛋白水平的差异。结果:NT-3和BDNF蛋白水平在大鼠3～4周龄和5～8周龄各有一次峰值,NT-4蛋白水平不具备这一时间特征;运动组NT-4以及NT-3和BDNF在3～5周龄的蛋白水平高于对照组,悬吊组则低于对照组。结论:NT-3和BDNF对NMJ在出生后早期和青春期的正常发育起重要诱导作用。NT-4则主要与NMJ神经肌肉活动依赖性功能发育有关;运动训练可以在成长期促进NT-4以及出生后早期NT-3和BDNF蛋白水平的升高,从而促进NMJ发育成熟。 Muscle-derived neurotrophins （NTs） play important roles in the development of neuromuscular junction （NMJ）. In this study, 3-week-old male Sprague-Dawley rats had undergone activity-enhancing （treadmill-running） and activity-reducing （tail-suspension） respectively to discuss the characteristics of neurotrophins concentrations in growth phase as well as the effects of activities. Results showed that NT-3 and BDNF presented peak concentrations in 3- 4 weeks and 5 -8 weeks after birth, which illuminated that they throw great effects on NMJ development both in early stage after birth and adolescence. The concentration of NT-4 was affected by activity-changing and had no relationship with age-changing, which suggested that it was related mainly to neuromuscular reliant function development. It was also approved that exercises could improve the concentrations of NT-4 as well as that of NT-3and BDNF in early stage after birth, which supported that training could promote the development of NMJ in growth stage.

作者安楠

机构地区国家体育总局体育科学研究所

出处《体育科学》 CSSCI 北大核心 2012年第7期53-57,共5页 China Sport Science

基金国家体育总局体育科学研究所基本科研业务费资助项目(基本06-17)

关键词神经营养因子神经肌肉接头成长期发育鼠动物实验 neurotrophin neuromuscular junction growth phase development rat animal ex-periment

分类号 G804.2 [文化科学—运动人体科学]

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