摘要
目的探讨miR-450a腺病毒对体外培养小鼠早期胚胎发育的影响。方法取小鼠2、4、8细胞期胚胎,用含不同浓度miR-450a腺病毒(0.01、0.1、1 ng/ml)的M16培养液培养,并设不含miR-450a腺病毒的对照组。倒置显微镜观察胚胎发育情况,并计算2、4、8细胞期的胚胎发育率。结果 miR-450a腺病毒可明显抑制受精卵细胞的正常分裂,导致小鼠胚胎发育异常,且随着miR-450a腺病毒浓度的增加,胚胎发育率明显下降(P<0.05),在较高浓度miR-450a腺病毒培养过程中,有部分胚胎死亡、退化。结论 miR-450a腺病毒对体外培养的小鼠早期胚胎发育具有抑制作用,为胚胎发育异常的研究提供了新的思路。
Objective To investigate the effect of miR-450a on development at early stage of mouse embryo cultured in vitro. Methods The mouse embryos at stages of 2 - 8 cells were cultured in M16 media containing 0.01, 0.1 and 1 ng/ml miR-450a re- spectively, using those in miR-450a-free medium as control. The development of mouse embryo was observed under inverted micro- scope, and the development rates of embryos at stages of 2, 4 and 8 cells were calculated. Results The miR-450a inhibited the normal division of embryonated egg cells significantly and caused the abnormal development of mouse embryos. The development rate of embryos decreased with the increasing concentration of miR-450a （P 〈 0. 05）. Partial embryos died or degenerated during culture in media containing high miR-450a concentrations. Conclusion The miR-450a showed inhibitory effect on development at early stage of mouse embryo cultured in vitro, which provided a novel route for study on abnormal development of embryo.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第7期859-861,共3页
Chinese Journal of Biologicals
基金
重庆市自然科学基金项目(CSTC
2011BB5131)
