摘要
目的研究补肾活血方对卵巢早衰(POF)小鼠卵泡凋亡调控相关基因的表达,为临床中医药预防和治疗卵巢早衰提供思路。方法采用透明带3作抗原,对BALB/c雌性小鼠皮下多点注射,建立免疫性POF模型。用补肾活血方低、中、高剂量进行治疗,设倍美力为阳性对照药,采用ELISA法测定外周血清抗透明带抗体(AzpAb)的含量,进行脏器指数及卵巢病理学观察,免疫组织化学SABC法检测卵泡凋亡调控基因(bcl-2/Bax)表达变化。结果与空白组比较,模型组AzpAb表达明显增高,差异有统计学意义(P<0.01);与模型组比较,各剂量组、倍美力组表达均明显下降,差异有统计学意义(P<0.01);与空白组比较,模型组卵巢指数、胸腺指数均明显下降,脾脏指数明显增加,差异有统计学意义(P<0.01),与模型组比较,高剂组和倍美力组卵巢指数(P<0.01)、胸腺指数(P<0.05)增加,脾脏指数降低(P<0.05);与空白组比较,模型组bcl-2蛋白表达明显下降、Bax蛋白表达显著增加,差异有统计学意义(P<0.01),与模型组比较,各剂量组、倍美力组bcl-2蛋白表达明显增高(P<0.01),Bax蛋白表达显著降低(P<0.01)。结论补肾活血方可能通过调节凋亡调控基因(bcl-2/Bax)的表达,使AzpAb表达明显下降,从而调节免疫反应,抑制抗体集聚,阻止卵泡的过度凋亡,改善卵巢的免疫功能,使有生机卵泡增加,淋巴细胞、浆细胞浸润减少,达到抑制自身免疫损伤、保护卵巢、改善卵巢功能的目的。
Objective To study the efffects of Bushen Huoxue decoction on premature ovarian failure（POF） of follicle apoptosis-related protein expression in mice and provide ideas for clinical prevention and treatment of POF with Chinese medicine.Methods The zona pellucida 3 as the antigen,the BALB/c female mice,were given multi-point injectionin subcutaneous immunization to induce the models of autoimmune POF.Then the mice were treated with Bushen Huoxue decoction in low-dose,medium-dose,high-dose and Premarin（as control）,respectively.Ovarian index,thymus index,spleen index and the morphology of ovary tissue were studied.The follicle apoptosis-related regulatory protein（Bcl-2/Bax） expression was detected by immunohistochemistry SABC and serum AzpAb content was measured by ELISA.Results Compared with model group,the ovarian index and thymus index were increased（P〈0.01,P〈0.05）,and spleen index was decreased（P〈0.05） in Bushen Huoxue decoction high-dose group and Premarin group;the ovarian index and thymus index were increased and spleen index was decreased in Bushen Huoxue prescription median-dose group and low-dose group,but there were no significant differences.Bcl-2 protein expression in the control group,Bushen Huoxue prescription all dose groups,Premarin group were significantly higher than that of modeling group（all P〈0.05）.While Bax protein and AzpAb expressions in the control group,Bushen huoxue decoction in all dose groups,Premarin group were significantly lower than that in model group（P〈0.05）.Conclusion Bushen Huoxue decoction may influence the apoptosis-related regulatory protein（such as Bcl-2 and Bax） expressions,decrease AzpAb expression to improve ovarian function.
出处
《湖南中医药大学学报》
CAS
2012年第5期28-31,42,共5页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金资助项目(30973771)
中国博士后科研基金(2012M511382)
湖南省自然科学基金资助项目(11JJ4075)
国家教育部高等学校博士学科点专项科研基金(20114323120007)
湖南省大学生研究性学习和创新性实验计划项目(2011-480)
关键词
补肾活血方
卵巢早衰
自身免疫
卵泡凋亡
抗透明带抗体
小鼠
补骨脂
紫石英
菟丝子
premature ovarian failure
Bushen Huoxue decoction
autoimmunity
apoptosis
AzpAb
mice
Psoralea corylifolia
Taxillus chinensis Danser
Cuscuta chinensis Lam
