摘要
目的:观察匹维溴胺对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型大鼠TNF-α含量变化的影响,探讨其治疗D-IBS的作用机制。方法:复制大鼠D-IBS模型,将40只大鼠随机分为正常组、模型组、安慰剂组和匹维溴胺组,每组10只,采用ELISA法和半定量RT-PCR法检测各组TNF-α表达的变化。结果:与正常组相比,模型组和安慰剂组大鼠结肠组织TNF-α含量明显增高(P<0.05);与模型组和安慰剂组相比,匹维溴胺组大鼠结肠组织TNF-α含量明显降低(P<0.05)。结论:匹维溴胺对D-IBS的治疗作用,其作用机制可能与降低TNF-α的表达有关。
Objective. To investigate the effects of pinaverium bromide on the expression of TNF-αin rats with diarrhea-predominant irritable bowel syndrome（D-IBS）. Methods. D-IBS animal model was established by stress combined with constraint. Forty rats were randomly divided into 4 groups： normal group, model group,vehicle group and pinaverium bromide group. The expression of TNF-α was detected by ELISA and RT-PCR. Results. Compared with the normal group,the expression of TNF-α was increased significantly in model group and vehicle group ; comparing with the model group and vehicle group,the expression of TNF-α was decreased significantly in pinaverium bromide group. Conclusion.. The pinaverium bromide can treat D-IBS,and this activity may be associated with decreasing the expressions of TNF-α in the colon tissue.
出处
《海南医学院学报》
CAS
2012年第7期877-879,共3页
Journal of Hainan Medical University
基金
中国高校医学期刊临床专项资金项目(112210210)~~
