阿托伐他汀对人脐静脉内皮细胞Lox-1、TNF-α及ICAM-1表达的影响被引量：1

Effect of atorvastatin on expression of LOX-1,TNF-α and ICAM-1 in human umbilical vein endothelial cells

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摘要目的研究阿托伐他汀(ATV)对脂多糖(LPS)诱导的人脐静脉内皮细胞血凝素样氧化低密度脂蛋白受体-1(LOX-1)、肿瘤坏死因子-α(TNF-α)及细胞间黏附分子(ICAM-1)表达的影响,探讨ATV防治动脉粥样硬化的机制及其抗炎作用。方法体外培养人脐静脉内皮细胞,用LPS刺激人脐静脉内皮细胞后,加入ATV干预24h,收集细胞,用荧光定量PCR方法测定LOX-1、TNF-α及ICAM-1mRNA的表达;用Western blotting法测定LOX-1、TNF-α及ICAM-1蛋白表达。结果用LPS刺激人脐静脉内皮细胞后,引起LOX-1、TNF-α及ICAM-1的高表达,与空白对照组比较差异有统计学意义(P<0.01),用ATV干预以后显著抑制LOX-1、TNF-α及ICAM-1的表达,与模型组比较差异有统计学意义(P<0.01)。结论 ATV可抑制LOX-1、TNF-α及ICAM-1的表达,这可能是其发挥抗动脉粥样硬化作用及抗炎作用的机制之一。 Objective To investigate the influence of atorvastatin on the expression of LOX-1,TNFa and ICAM-1 in human umbilical vein endothelial cells （HUVECs） ,and to study its possible anti-atherosclerotic mechanism. Methods HUVECs were cultured in vitro and stimulated with LPS, then treated with atorvastatin for 24 h, finally measured the expression of LOX-1 ,TNF-a and ICAM-1 mRNA with real-time PCR. Western blotting method was used to analyze the expression of LOX 1, TNF-a and ICAM-1 protein. Results LPS enhanced the expression of LOX-1,TNF-a and ICAM-1, with statistical difference compared with the normal control group（P〈0.01）. Atorvastatin decreased the high expression of LOX-1, TNFu and ICAM-1, showing statistical difference compared with the model group（P〈0.01）which were stimulated by LPS. Conclusion Atorvastatin can block the high expression of LOX-1,TNF-a and ICAM-l,which may be one of the mechanisms to play the anti-artherosclerosis and anti-inflammation role.

作者姜玉姬姜华

机构地区延边大学附属医院肾内科

出处《重庆医学》 CAS CSCD 北大核心 2012年第17期1708-1709,1712,共3页 Chongqing medicine

基金国家自然科学基金资助项目(81160476)

关键词 ATV 内皮细胞 LOX- TNF-Α ICAM-1 动脉粥样硬化 atorvastatin HUVECs LOX-1, TNF-a ICAM-1 artherosclerosis

分类号 R96 [医药卫生—药理学]

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参考文献9

1Hansson GK. Atherosclerosis an immune disease:The Anitschkov Lecture 2007[J].Atheroclerosis,2009,(01):2-10.doi:10.1016/j.atherosclerosis.2008.08.039.
2王虹艳,曲鹏,富晶,姜华.TLR4激动上调内皮细胞氧化低密度脂蛋白受体LOX-1表达[J].高血压杂志,2005,13(7):422-426. 被引量：10
3姜华,张艳,王辰.益气活血复方含药血清对人脐静脉内皮细胞TLR4及其下游髓样分化因子88、肿瘤坏死因子受体相关因子-6表达的影响[J].中国中西医结合杂志,2010,30(5):519-522. 被引量：15
4Koga T,Claycombe K,Meydani M. Homocysteine increases monocyte and T cell adhesionto human aortic endothelial cells[J].Atheroclerosis,2002,(02):365-374.doi:10.1016/S0021-9150(01)00670-0.
5Papa A,Danese S,Kondeatis. Intercellular adhesion molecule-1 gene polymorphisms in inflammatory bowel disease[J].European Review for Medical and Pharmacological Sciences,2004,(05):187-191.
6Meroni PL,Raschi E,Testoni C. Statins prevent endothelial cell activation induced by antiphospholipid(anti-beta2-glycoprotein I)antibodies:Effect on the proadhesive and proinflammatory phenotype[J].Arthritis and Rheumatism,2001.2870-2878.doi:10.1002/1529-0131(200112)44:12<2870::AID-ART475>3.0.CO;2-Y.
7Reiss A B,Anwar K,Wirkowski P. Lectin-like oxidized low density lipoprotein receptor 1(LOX-1) in atherogenesis:a brief review[J].Current Medicinal Chemistry,2009,(21):2641-2652.doi:10.2174/092986709788681994.
8Mehta JL,Chen J,Yu F. Aspirin inhibits ox-LDL-mediated LOX-1 expression and metalloproteinase-1 in human coronary endothelial cells[J].Cardiovascular Research,2004,(02):243-249.doi:10.1016/j.cardiores.2004.07.002.
9Hoffmann R,Haager P,Suliman H. Effect of statin therapy before Q-wave myocardial infarction on myocardial perfusion[J].American Journal of Cardiology,2008.139-143.doi:10.1016/j.amjcard.2007.07.059.

二级参考文献22

1Ross R.Atherosclerosis--an inflammatory disease.[J].N Engl J Med,1999,340(2):115-126.
2Kiechl S,Wiedermann CJ,Willeit J.Toll-like receptor 4 and atherogenesis[J].Ann Med,2003,35(3):164-171.
3Zeuke S,Ulmer AJ,Kusumoto S,et al.TLR4-mediated inflammatory activation of human coronary artery endothelial cells by LPS[J].Cardiovasc Res,2002,56(1):126-134.
4Edfeldt K,Swedenborg J,Hansson GK,et al.Expression of toll-like receptors in human atherosclerotic lesions:a possible pathway for plaque activation[J].Circulation,2002,105(10):1158-1161.
5Michelsen KS,Wong MH,Shah PK,et al.Lack of Tolllike receptor 4 or myeloid differentiation factor 88 reduces atherosclerosis and alters plaque phenotype in mice deficient in apolipoprotein E[J].Proc Natl Acad Sci USA,2004,101(29):10679-10684.
6Netea MG,van Deuren M,Kullberg BJ,el al.Does the shape of lipid A determine the interaction of LPS with Tolllike receptors?[J].Trends Immunol,2002,23(3):135-139.
7赵保胜,刘洪斌,马悦颖,隋峰,李兰芳,郭淑英,何希荣,霍海如,姜廷良.桂枝汤含药血清对小鼠巨噬细胞Toll样受体3、4型及其下游信号转导通路元件的影响[J].中药药理与临床,2007,23(3):1-4. 被引量：30
8Xu XH, Shah PK, Faure E, et al. Toll-like receptor-4 is expressed by macrophages in murine and human lipid-rich atherosclerotic plaques and upregulated by oxidized LDL[J]. Circulation, 2001,104: 3103-3108.
9Edfeldt K, Swedenborg J, Hansson GK, et al. Expression of toll-like receptors in human atherosclerotic lesions: a possible pathway for plaque activation[J]. Circulation, 2002,105: 1158-1161.
10Shi W, Haberland ME, Jien ML, et al. Endothelial responses to oxidized lipoproteins determine genetic susceptibility to atherosclerosis in mice[J]. Circulation, 2000,102: 75-81.