摘要
目的:研究奥卡西平(Oxcarbazepine,OXC)对大鼠三叉神经节神经元钙电流的调控作用。方法:SD大鼠随机分为3组(n=8):生理盐水组(NS组),致炎剂组(IS组),OXC预防组。应用膜片钳技术,采用全细胞记录方式,观察OXC对偏头痛大鼠急性分离的三叉神经节的高电压激活钙电流(HVA-ICa)的调控作用。结果:OXC能够抑制钙电流,使钙电流的激活曲线向去极化方向移动,使钙电流的失活曲线向超级化方向移动。结论:OXC可能通过抑制钙离子进入细胞膜,来预防偏头痛的发作。同时OXC可能对外周神经系统及伤害感受的传入的兴奋性起到调控作用。
Objective： The aim of the present study was to investigate the effects of Oxcarbazepine（OXC） on high voltage active calcium currents in trigeminal ganglion neurons in rat model of migraine.Methods： Male adult Sprague Dawley rats were randomly di-vided into 3 groups（each group n=8）： normal saline treatment group,IS infusion group,OXC prevention group.We report the effects of OXC on calcium currents,using a conventional whole-cell patch clamp technique fromacutely isolated rat trigeminal ganglion neurons.Results： OXC inhibited calcium currents.Moreover,OXC could shift the voltage-gated activation curve for calcium channel in the depo-larizing direction;shift the steady-state inactivation curve for calcium channel in the hyperpolarizing direction.Conclusion： OXC,whose preventative action in migraine prophylaxis may involve blockade of Ca2＋ entry across the cell membrane,prevent migraine efficiently.Considering its complex modulatory effects on voltage-gated calcium channels,OXC might have potential effects on the nervous system and lead to a change in excitability of nociceptive afferent fibers.
出处
《现代生物医学进展》
CAS
2012年第13期2474-2477,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81070896)
关键词
奥卡西平
偏头痛
三叉神经节神经元
全细胞膜片钳
钙电流
Oxcarbazepine
Migraine
Trigeminal ganglion neurons
Whole cell patch clamp
Calcium currents
