摘要
目的 探讨微丝在碱性成纤维细胞生长因子 (bFGF)调节伤口愈合中的作用。 方法 采用免疫荧光染色、显微荧光光度计及 3H -TdR掺入法研究bFGF诱导的成纤维细胞微丝骨架的变化 ,观察用细胞松弛素B破坏微丝后 ,bFGF对细胞运动及细胞增殖的影响。 结果 bFGF使成纤维细胞微丝骨架发生有序的变化 ;使F -肌动蛋白 (F -actin)含量增高 ,并呈一定的时间 -剂量依赖关系。用细胞松弛素B破坏微丝 ,可明显抑制由bFGF引起的细胞向创面的迁移 ,同时也显著抑制了bFGF引起的成纤维细胞 3H -TdR掺入量。 结论 bFGF可通过微丝骨架的变化影响创伤愈合过程中细胞的迁移、分化和增殖。
Objective To investigate the effects of microfilaments on basic fibroblast growth factor in (bFGF) regulating wound healing. Methods With immunofluorescence microscopy, microfluorometry and 3 H TdR incorporation methods,the changes of fibroblast microfilaments induced by bFGF were observed, and the effects of bFGF on cell migration and proliferation were studied after the disruption of microfilaments with cytochalasin B. Results The exposure of fibroblasts to bFGF caused significant changes in microfilaments. Quantitative assay showed that bFGF could result in a rapid time and dose dependent increase in F actin content. The disruption of microfilaments with cytochalasin B significantly inhibited the cell migration and 3 H TdR incorporation induced by bFGF. Conclusions It suggests that bFGF takes action through microfilament mediated mechanism. The microfilament cytoskeleton may be crucial to the regulation of growth factors during cell migration, division and differentiation, which are fundamental to wound healing.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2000年第6期331-333,共3页
Chinese Journal of Trauma
关键词
细胞骨架
BFGF
细胞外基质
创伤愈合
微丝
Cytoskeleton
Fibroblast growth factor, basic
Extracellular matrix
Wound healing
