摘要
华蟾素是从中华大蟾蜍Bufo bufo gargarizansCantor的干燥皮提取精制而成的水溶性制剂,已列为国家中药保护品种。其注射液是应用较早的剂型之一,目前广泛应用于多种中晚期肿瘤,如原发性肝癌、胃癌、肺癌、结肠癌、胰腺癌等[1？3]。大量的临床与实验研究证实华蟾素注射液是安全、
Cinobufacini is an aqueous extract of Bufo bufo gargarizans Cantor dried skin,which has been widely used for cancer therapy in China.So far,its active components are still not very clear.In previous reports,bufadienolides with low-concentration were usually studied because of their anticancer effects.However,the high polarity constituents in cinobufacini are less investigated.The present study found that more than 50% contents of cinobufacini were water-soluble peptides.Then,in vitro anticancer experiments were carried out,including human stomach cancer cell lines BGC823 and MCG803,human colon cancer cell lines DLD-1 and HT-29,and human pancreatic cancer cell line MIAPACA-2.The IC50 for these cell lines model were ranged from 25-123 -g·mL/1.The results indicated that these peptides showed similar activity with cinobufacini injection.As a conclusion,this study provides a new and further understanding of anticancer components in cinobufacini injection.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第6期822-826,共5页
Acta Pharmaceutica Sinica
基金
中国中医科学院自选课题资助项目(z02063)
关键词
华蟾素注射液
多肽组分
抗肿瘤
抑制增殖
cinobufacini injection
peptide composition
anticancer
anti-proliferation
