摘要
背景:神经干细胞为神经发育和神经功能重建的研究带来了广阔前景,其体外培养已成为神经科学的一个研究基础。目的:比较胶原酶和胰酶对体外培养神经干细胞的作用。方法:取孕14d的SD大鼠,无菌条件下取胎鼠端脑,剪碎后分别用含EDTA的胰酶和Ⅰ型胶原酶消化,无血清培养基培养细胞。结果与结论:胶原酶消化得到的神经干细胞呈单层贴壁生长,而用胰酶消化得到的则形成聚集体;Nestin免疫荧光鉴定细胞为阳性;获取的神经干细胞纯度大于99%。提示用胶原酶可以简单而快速地获得原代单层化贴壁生长的神经干细胞。
BACKGROUND：Neural stem cells（NSCs） bring wide prospect for the study of neural development and repairing,and the in vitro culture is foundational to neuroscience.OBJECTIVE：To contrast the effects of trypsogen and collagenase on culture of NSCs in vitro.METHODS：The fetal rat telencephalons were isolated from SD rats pregnant for 14 days under sterile conditions,and tissues were divisively treated with trypsogen and typeⅠ collagenase contained EDTA following trituration,and cultured with serum-free medium.RESULTS AND CONCLUSION：The adherent monoculture NSCs were obtained using collagenase and congeries of cells were obtained using trypsogen.Nestin immunofluorescence identification showed the cells were positive,and the purity was over 99%.The primary adherent monoculture NSCs can be simply and quickly got using collagenase.
出处
《中国组织工程研究》
CAS
CSCD
2012年第14期2563-2566,共4页
Chinese Journal of Tissue Engineering Research
基金
黑龙江省普通高等学校青年骨干支持项目(1155G60)~~
