摘要
目的:研究不同配方及浓度的胰蛋白酶对人瘢痕角质形成细胞(Ker at i nocyt e,K)生物活性影响。探索一种更适于瘢痕角质形成细胞原代培养消化的方法。方法:温消化法分离瘢痕组织表皮、真皮后,分别采用0.1%胰蛋白酶和0.05%胰蛋白酶-0.025%EDTA对表皮片进行消化,培养角质形成细胞,用台盼蓝染色法和24h细胞贴壁率观察K存活率及细胞活性。结果:两种配方胰酶消化K,细胞存活率均达80%以上,结果无统计学差异(P>0.05)。与单独使用胰蛋白酶相比,经胰蛋白酶-EDTA组消化后的细胞贴壁率增加,结果有统计学差异(P<0.05)。结论:胰蛋白酶-EDTA能较温和地消化角质形成细胞,较大程度地保留细胞活性,与胰蛋白酶消化法相比,此方法更适合K的消化及培养。
Objective To research the effect of different digestion methods acted on keratinocytes and obtain a better one. Methods Cultured keratinocytes derived from hypertrophic scar using digestion 0.1% trypase and 0.05% trypase with 0.025% ethlenediamine tetracetic acid （EDTA）.Cell vigor was observed with teypane blue stained and calculated the adherence rate in 24 hours. Results Keratinocytes digested by different trypase showed over 80% survival rate. However,a significant difference was found on adherence rate in 24 hours （P〈 0.05）. Conclusion Digestion with 0.05% trypase mixed 0. 025% EDTA was better for cultivation of keratinocyte compared with 0.1%trypase using alone.
出处
《中国美容医学》
CAS
2012年第5期762-763,共2页
Chinese Journal of Aesthetic Medicine
