摘要
目的:探讨艾灸预处理对运动员大负荷训练阶段运动性免疫机能失衡的防治作用机制。方法:12名男子中长跑运动员,随机分为对照组(6名)和观察组(6名)。在大负荷训练阶段,对照组只训练,观察组在每天下午训练后灸关元、足三里穴(双)或关元、三阴交穴(双),每天灸一组,两组穴位交替治疗。分别在大负荷运动训练前、大负荷运动训练3周后、调整训练1周后采用实时荧光定量PCR技术检测外周血白细胞转录因子T-bet(T-box expressed in T cells)和GATA-3(GATA binding protein 3)mRNA的表达。结果:(1)两组运动员T-bet mRNA的表达均表现为上升的趋势,对照组上升了4.52%,观察组上升了14.8%,但两组间和组内比较差异均无统计学意义(P>0.05)。(2)GATA-3mRNA表达,对照组调整训练后与大负荷训练前和大负荷训练后相比均明显上升(P<0.05),观察组调整训练后与同期对照组相比显著下降(P<0.05)。(3)T-bet、GATA-3mRNA比值,对照组大负荷训练后比大负荷训练前增高了13.58%,调整训练后比大负荷训练前降低了29.63%,整体上呈现下降趋势;观察组大负荷训练后比大负荷训练前增高75.16%,调整训练后比大负荷训练前仍增高35.4%,整体上呈现上升趋势。结论:(1)中长跑运动员调整训练后GATA-3mRNA明显增高,上调T辅助淋巴细胞(Th)2型细胞因子产生,导致了Th 1/Th 2平衡向Th 2漂移;(2)艾灸预处理抑制了运动员大负荷训练后GATA-3mRNA过度表达,可能是维持运动员Th 1/Th 2动态平衡的机制之一。
Objective To observe the effect of moxibustion pretreatment on the expression of leukocytic T-box transcription factor expressed in T cells（T-bet） mRNA and trans-acting T-cell-specific transcription factor GATA-3 mRNA in athletes undergoing heavy load training,so as to study its mechanism underlying prevention of imbalance of immune function.Methods Twelve male middle-long distance running athletes were equally randomized into control group and treatment group.The althelets in the treatment group accepted alternate moxibustion pretreatment of Guanyuan（CV 12）＋bilateral Zusanli（ST 36） or Guanyuan（CV 12）＋ bilateral Sanyinjiao（SP 6） for 30 min,once a day for 4 weeks,and beginning from the 1st day of the heavy load trai-ning on till the end of the modulatory training.The althletes of the control group were asked to conduct simple heavy load running training.Leukocytes in the collected venous blood samples were acquired following erythrocyte lysis,centrifugation and removal of the supernatant.The expression levels of leukocytic T-bet mRNA and GATA-3 mRNA were detected before,3 weeks after the heavy load running training and 1 week after the adjustment training.Results In comparison with pre-training,the expression levels of leukocytic T-bet mRNA were increased slightly after heavy load running training（7.24%,16.84%） and after adjustment training（4.52%,14.8%） in both control and treatment groups（P0.05）.After heavy load running training,the expression le-vels of leukocytic GATA-3 mRNA were dow-regulated mildly（13.14%,34.04%） in both control and treatment groups（P0.05）,but upregulated considerably in the control group（59.12%,P0.05） rather than in the treatment group（-17.02%,P0.05）.The GATA-3 mRNA expression level of the treatment group was significantly lower than that of the control group（P0.05）.The ratios of leukocytic T-bet mRNA/GATA-3 mRNA were increased by 13.58% and 75.16% after heavy load running training and decreased by 29.63% and increased by 35.4%,respectively after adjustment training in the control and treatment groups in comparison with pre-training.No significant differences were found between two groups in the expression levels of T-bet mRNA and the ratios of T-bet mRNA/GATA-3 mRNA at the 3 time-points,and in the expression levels of GATA-3 mRNA before and after heavy load running training（P0.05）.Conclusion Moxibustion pretreatment can inhibit the expression of leukocytic GATA-3 mRNA after adjustment training in the middle-long distance heavy load running athletes,which may contribute to its effect in regulating imbalance of Th 1/Th 2 after heavy load training.
出处
《针刺研究》
CAS
CSCD
北大核心
2012年第2期125-130,共6页
Acupuncture Research
基金
中国博士后科学基金资助项目(20100470718
201104278)
上海市体育局腾飞计划资助项目(10TF009)
上海市教委第五期重点学科建设资助项目(J51001)
上海市教委预算内资助项目(2010JW09)
