摘要
目的研究唑来膦酸对去势大鼠骨质疏松性骨折愈合及转化生长因子(TGF-β)表达的影响。方法 60只6月龄雌性SD大鼠建立骨质疏松性骨折模型后,将其随机分为实验组和对照组,分别给予唑来膦酸、生理盐水皮下注射。两组于术后不同时间段取骨痂行苏木素-伊红(HE)染色,测骨密度、免疫组化及X线片观察骨折愈合情况。结果实验组比同期对照组骨痂生成量多、愈合时间提前、骨折局部骨密度明显增高,差异有统计学意义(P<0.05)。TGF-β于4周左右表达达到高峰,实验组比对照组TGF-β表达更明显(P<0.05)。结论唑来膦酸能加快骨痂形成,促进成骨细胞TGF-β的表达,促进骨质疏松性骨折的愈合。
Objective To research the effects of zoledronic acid on healing of osteoporotic fractures and expression of TGF-β in rats. Methods Sixty 6-month-old female SD rats of osteoporotic fracture model were randomly divided into control group and experimental group, and were given saline injection or zoledronic acid respectively. The callus formation was assessed with HE-stained histology; The healing of osteoporotic fractures was assessed by TGF-β immunohistochemistry （IHC）, bone mineral density （BMD） and X-ray. Results Compared with the concurrent control group, the experimental group had more callus formation, more advanced healing time, markedly higher bone mineral density in fracture region, and the differences were statistically significant （P 〈 0.05）. TGF-β expression reached its peak at about four weeks, and TGF-β expression of the experimental group was more than that of the control group （P 〈 0.05）. Conclusion Zoledronic acid can stimulate the growth of bone, promote the healing of osteoporotic fracture through promoting expression of TGF-β in ovariectomized rats.
出处
《中国现代医生》
2012年第9期13-14,17,共3页
China Modern Doctor
