摘要
以牛喉管为原料,采用超声波辅助碱-双酶(木瓜蛋白酶、胃蛋白酶)法提取硫酸软骨素。通过单因素试验、正交试验优化胃蛋白酶酶解硫酸软骨素的工艺。结果显示:当pH值为5.9、胃蛋白酶添加量为1.4:1000(m/V)、酶解温度40℃、酶解时间为100min时,产品最高得率可达25.43%,纯度达到87.96%。
Ultrasonic pretreatment followed by sequential hydrolysis with papain and pepsin was used to extract chondroitin sulfate from bovine pipes.The optimal pepsin hydrolysis conditions were identified by one-factor-at-a-time and orthogonal array design methods to be pH 5.9,pepsin dosage 1.4：1000（m/V）,temperature 40 ℃ and hydrolysis time 100 min.Under these conditions,the extraction yield of chondroitin sulfate was 25.43% with a purity of 87.96%.All quality indicators investigated completely reached the requirements of relevant national standards for chondroitin sulfate.
出处
《肉类研究》
2011年第8期22-25,共4页
Meat Research
基金
国家现代农业(肉牛
耗牛)产业技术体系建设专项(CARS-38)
