摘要
本试验旨在研究低温环境下饲粮核黄素添加水平对蛋鸭生长发育、免疫器官及抗氧化功能的影响。试验采用两因素有重复完全交叉设计,温度设2个水平[适温(18±1)℃,T1;低温(2±1)℃,T2];核黄素设3个水平,在基础饲粮中分别添加4、16、32mg/kg核黄素。选用12周龄健康的金定鸭(母)144只,随机分成6个处理,每个处理6个重复,每个重复4只,试验期为42d。结果表明:低温环境和核黄素互作对卵巢重量指数和输卵管长度指数均有显著影响(P<0.05),但对输卵管重量指数没有显著影响(P>0.05)。低温环境和核黄素互作对各免疫器官指数均有显著影响(P<0.05),但对血清中总抗氧化能力、谷胱甘肽还原酶活性和丙二醛浓度没有显著影响(P>0.05),对过氧化氢酶和总超氧化物歧化酶活性有显著影响(P<0.05)。由此可见,低温环境不利于蛋鸭的生长和发育,适当提高饲粮核黄素添加水平可以在一定程度上缓解机体对低温环境产生的应激反应。
The experiment was conducted to study the effects of riboflavin supplementation on growth and development,immune organ and anti-oxidative ability of laying ducks at low ambient temperature.Two factors(temperature×riboflavin) interactive experimental design was used in the experiment.The ambient temperatures were(2±1) ℃ and(18±1) ℃,and supplemental levels of riboflavin in a basal diet were 4,16 and 32 mg/kg,respectively.A total of 144 laying ducks(Jinding duck,female,12 weeks old) were randomly allotted to 6 treatments with 6 replicates per treatment and each replicate comprised 4 ducks.The experiment lasted for 42 days.The results showed that ovary weight index and oviduct length index were significantly affected by the interaction between ambient temperature and riboflavin(P0.05),however,the interaction between ambient temperature and riboflavin has no effect on the oviduct weight index(P0.05).Immune organ indices were significantly affected by the interaction between ambient temperature and riboflavin(P0.05).There were no significant differences in serum total antioxidant capacity,the activities of glutathione reductase and malondialdehyde concentration(P0.05).However,catalase and total superoxide dismutase activity were significantly affected by the interaction between ambient temperature and riboflavin(P0.05).In conclusion,low ambient temperature is not conducive to growth and development of laying ducks,and appropriately increasing dietary riboflavin level in diets can release cold stress response to a certain extent.
出处
《动物营养学报》
CAS
CSCD
北大核心
2011年第11期1912-1918,共7页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然基金委资助(30972111)
关键词
寒冷应激
核黄素
免疫
抗氧化
蛋鸭
cold stress
riboflavin
immune
anti-oxidation
laying ducks