摘要
目的:研究枳椇子醋酸乙酯提取物抗细胞氧化的作用机制。方法:对枳椇子醋酸乙酯部位进行初步分离得到5个组分,进行体外抗氧化实验。将培养好的不同细胞分为空白对照组,阳性对照组(加入H2O2诱导产生活性氧),实验组(加入H2O2和枳椇子醋酸乙酯部位的5个组分,分别为实验组A、B、C、D、E)。通过检测细胞内荧光强度进行细胞内活性氧实验和细胞增殖实验。同时提取各组细胞蛋白,运用Western-blot法检测各实验组细胞内p-JNK蛋白的表达。结果:细胞内活性氧实验中对人正常肝上皮细胞LO2抑制效果最明显的是实验组B,对肝癌细胞HepG2抑制效果最明显的是实验组C;细胞增殖实验中对人正常肝上皮细胞LO2促进效果最明显的是实验组A;Western-blot法实验中能使肝癌细胞HepG2中p-JNK蛋白表达降低的为实验组B。结论:体外细胞实验证实枳椇子醋酸乙酯部位不同组分可通过抑制细胞内活性氧的产生而抑制细胞凋亡,从而促进肝细胞增殖。
OBJECTIVE To study the cell mechanism of anti-oxidation of ethyl acetate extract from the seeds of Hovenia acerba Lindl.. METHODS Ethyl acetate extract from the seeds of Hovenia acerba Lindl. was divided into 5 components undergoing anti-oxidation experiment in vitro. The cell was divided into blank control group, positive control group with H2O2 and experimental group A, B, C, D, E with H2O2 and 5 components from ethyl acetate extract of the seeds of Hovenia acerba Lindl.. The intracellular reactive oxygen species and cell proliferation experiments were done by detecting the fluorescence intensity. The intracellular p-JNK protein from different experimental groups was detected by Western-blot. RESULTS The experimental group B and C had the most obvious inhibitory effect on normal human liver epithelial cell LO2 and HepG2 respectively in reactive oxygen species experiment. The experimental group A had the most obvious promoting effect on normal hu- man liver epithelial cell LO2 in ceil proliferation experiment. The p-JNK protein expression in the HepG2 was inhibited by the experimental group B in the Western-blot experiment. CONCLUSION The generation of intracellular reactive oxygen species was inhibited by ethyl acetate extract from the seeds of Hovenia acerba Lindl. in the experiment in vitro, promoting cell proliferation.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2012年第8期617-620,654,共5页
Chinese Journal of Hospital Pharmacy
