摘要
利用野油菜黄单胞菌(Xanthomonas campestris pv.campestris)8004胞内粗酶液生物催化合成α-熊果苷,考察了对苯二酚浓度、反应物摩尔比、缓冲溶液pH值、反应时间、菌体浓度等因素对反应的影响。确定最佳反应条件为:反应温度35℃、摇床转速180r.min-1、对苯二酚浓度40mmol.L-1、对苯二酚与蔗糖的摩尔比1∶30、缓冲溶液pH值7.0、反应时间36h、菌体浓度80mg.mL-1,在此条件下,α-熊果苷含量达到6.58mg.mL-1、对苯二酚选择性为66%、对苯二酚转化率为91%。
Biocatalytic synthesis of α-arbutin by the intracellular crude enzyme from Xanthomonas campestris pv.campestris 8004 was studied.The effects of hydroquinone concentration,reactant molar ratio,buffer solution pH value,reaction time and cell concentration on the reaction were investigated.The results showed that the optimum reaction conditions were as follows：the reaction temperature was 35 ℃,the rotational speed was 180 r·min-1,hydroquinone concentration was 40 mmol·L-1,the molar ratio of hydroquinone to sucrose was 1∶30,the buffer solution pH value was 7.0,the reaction time was 36 h,the cell concentration was 80 mg·mL-1.Under above conditions,α-arbutin content reached 6.58 mg·mL-1,the selectivity of hydroquinone was 66%,and the conversion rate of hydroquinone was 91%.
出处
《化学与生物工程》
CAS
2012年第4期64-67,共4页
Chemistry & Bioengineering
关键词
胞内粗酶液
α-熊果苷
选择性
转化率
the intracellular crude enzyme
α-arbutin
selectivity
conversion rate
