摘要
AIM:To evaluate for the first time the protein and mRNA expression of 14-3-3εin gastric carcinogenesis.METHODS:14-3-3εprotein expression was determined by western blotting,and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.RESULTS:Authors observed a significant reduction of 14-3-3εprotein expression in gastric cancer(GC)samples compared to their matched non-neoplastic tissue.Reduced levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this pathology.Our data suggest that reduced 14-3-3εmay have a role in gastric carcinogenesis process.CONCLUSION:Our results reveal that the reduced 14-3-3εexpression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcino-genesis process.
AIM： To evaluate for the first time the protein and mRNA expression of 14-3-3ε in gastric carcinogenesis. METHODS： 14-3-3ε protein expression was deter- mined by western blotting, and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples, RESULTS： Authors observed a significant reduction of 14-3-3ε protein expression in gastric cancer （GC） sam- ples compared to their matched non-neoplastic tissue. Reduced levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this pathology. Our data suggest that reduced 14-3-3ε may have a role in gastric carcinogenesis process, CONCLUSION： Our results reveal that the reduced 14-3-3ε expression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcinogenesis process.
基金
Supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq
Chammas R,Smith MAC and Burbano RR)
Fundao de Amparoà Pesquisa do Estado de So Paulo (FAPESP
Leal MF and Calcagno DQ)
