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黄连素对巨噬细胞清道夫受体表达的影响

Effect of berberine on the expression of scavenger receptors in macrophages
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摘要 目的:探讨黄连素(berberine)对巨噬细胞清道夫受体CD36、LOX-1、CD68等表达的影响。方法:以佛波醇酯(PMA)诱导分化的单核源巨噬细胞为细胞模型,黄连素(50μmol/L)预处理1 h后,加入氧化低密度脂蛋白(oxLDL)刺激,采用流式细胞仪检测巨噬细胞表面清道夫受体的表达。结果:单核细胞分化成巨噬细胞后,CD36表达明显升高,而黄连素预处理显著抑制CD36的表达。oxLDL刺激巨噬细胞24 h后,巨噬细胞表面清道夫受体CD36、LOX-1和CD68表达明显升高(P<0.01),黄连素预处理后巨噬细胞表面CD36、LOX-1的表达受到抑制,但CD68的表达未受影响(P>0.05)。结论:黄连素抑制巨噬细胞表面清道夫受体CD36、LOX-1的表达,一定程度上可减少巨噬细胞通过清道夫受体途径对脂质oxLDL的吞噬。 Objective： To investigate the effect of berberine on the expression of scavenger receptors（CD36,LOX-1,CD68） in macrophages.Methods： PMA-induced macrophages stimulated by oxLDL was used as model in this study to investigate the expression of scavenger receptors,including CD36,CD68 and LOX-1,which was tested using Flow cytometry.Results： Berberine greatly inhibited CD36 expression during monocytes differentiation to macrophages and similar result was obtained in oxLDL-stimulated macrophages.Moreover,in oxLDL-stimu-lated macrophages,LOX-1 expression,but not CD68,was also decreased after berberine treat-ment than those in oxLDL only group.Conclusion： Berberine suppresses the expression of CD36 and LOX-1,but not CD68 in macrophages.
作者 黄周青 蔡雪黎 卢中秋 黄伟剑
机构地区 温州医学院附属第一医院心血管内科 温州医学院附属第一医院急诊科
出处 《温州医学院学报》 CAS 2012年第2期107-109,共3页 Journal of Wenzhou Medical College
基金 浙江省中医药科技局科研基金资助项目(2010ZA085) 浙江省医学创新学科建设计划项目(11-CX26)
关键词 黄连素 清道夫受体 巨噬细胞 berberine scavenger receptors macrophages
分类号 R5 [医药卫生—内科学]
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参考文献16

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温州医学院学报
2012年 第2期

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