摘要
主要通过使用RPMI 1640培养基培养淋巴细胞、瑞氏—吉姆萨染色、DNA和RNA电泳等方法来研究棉酚对多浪羊淋巴细胞的凋亡效应和对淋巴细胞核酸影响。研究结果表明,经瑞氏—吉姆萨染色4 h淋巴细胞出现凋亡小体;DNA和RNA通过琼脂糖凝胶电泳检测在4 h分别出现梯状条带和18S降解。可见,当细胞培养液中棉酚浓度为1.35μmol/L,培养淋巴细胞4 h后,可抑制多浪羊淋巴细胞的增殖,并且促进凋亡及其核酸的降解。
In this study,the effects of gossypol on Duolang sheep lymphocyte apoptosis and degradation of nucleic acid on were studied by culturing lymphocytes in RPMI 1640 medium,Wright-Giemsa staining,DNA and RNA gel electrophoresis.The results showed that： lymphocyte appeared apoptotic bodies after Wright-Giemsa stained for 4 h;DNA and RNA were detected by Agarose Gel Electrophoresis.The DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis.The RNA agarose gel electrophoresis appeared 18S rRNA began to degrade at 4th hours.It was concluded that after treated by 1.35 μmol/L of gossypol for 4 h,the lymphocytes could inhibit lymphocyte proliferation of Duolang sheep,and promote apoptosis and degradation of nucleic acid.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第2期299-302,共4页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然基金项目(30960277)
关键词
棉酚
多浪羊
淋巴细胞
核酸
凋亡
gossypol
Duolang sheep
lymphocyte
gene
apoptosis
