摘要
目的 探讨人肺癌中nm2 3等位基因缺失与肺癌转移的关系。方法 应用Southern印迹杂交对 5 2例人肺癌组织中nm2 3 H1 和nm2 3 H2 等位基因缺失进行了研究 ,并以自身远离癌灶的肺组织作对照。结果 5 2例肺癌中 14例存在nm2 3 H1 等位基因的杂合缺失 ( 2 6.92 % )。 47例有nm2 3 H2 杂交信号的肺癌中 ,2例存在nm2 3 H2 等位基因缺失 ( 4 .2 6% )。伴有淋巴结和 /或远处转移的肺癌中 ,nm2 3 H1等位基因缺失率 ( 4 2 .86% )明显高于不伴有转移的肺癌 ( 8.3 3 % ) (P <0 .0 1) ;低分化和未分化癌nm2 3 H1等位基因缺失率 ( 4 5 .45 % )亦明显高于中～高分化癌 ( 13 .3 3 % ) (P <0 .0 5 )。nm2 3 H1 等位基因缺失与肺癌组织学类型、P TNM分期、原发肿瘤大小、部位 ,以及患者年龄等无明显关系 (P >0 .0 5 )。结论 本研究结果表明nm2 3基因可能参与调控肺癌的细胞分化和转移过程 ,且nm2 3 H1 基因在肺癌转移和细胞分化中的调节作用较nm2 3 H2
Objective To explore the role of allelic deletions of nm23 genes in human lung cancer. Methods Allelic deletions of nm23 genes were detected in 52 lung cancer tissue samples and their corresponding normal tissues as control by Southern blotting. Results Loss of heterozygosity (LOH) of nm23 H1 gene was found in 14 out of 52 informative lung cancers, with a LOH rate of 26.92%; 2 of the 47 informative lung cancer tissues existed allelic deletion of nm23 H2 gene, with a allelic deletion rate of 4.26%. The LOH rate of nm23 H1 gene in cancer with lymph node or distant metastasis(42.86%) was significantly higher than that in lung cancer without metastasis (8.33%) (P<0.01). nm23 H1 LOH rate was also remarkably higher in undifferentiated and poor differentiated cancer (45.45%) than in moderate to well differentiated cancer (13.33%) (P<0.05). No relationship was found among the allelic deletion of the nm23 H1 and histoclassification of the tumor, P TNM stages, size of the primary tumor, location of the cancer, and age of the patients (P>0.05). Conclusion nm23 gene may be involved in the differentiation and metastasis of human lung cancer, and nm23 H1 gene may play a more important role in the regulation of cell differentiation and metastasis of lung cancer than nm23 H2 gene.
出处
《中国肺癌杂志》
CAS
2000年第1期8-13,共6页
Chinese Journal of Lung Cancer
基金
国家自然科学基金 !(39470 687)
卫生部优秀青年科技人才专项基金! (Q9436)
美国纽约中华医学基金!(Y931 6)资助
关键词
NM23基因
肺肿瘤
等位基因缺失
nm23 gene Lung neoplasms Allelic deletion Southern blotting
