摘要
亲水作用色谱(HILIC)是一种分离极性和亲水性化合物的液相色谱模式,其作为反相液相色谱(RPLC)的重要补充,近年来越来越受到各个领域的关注和重视。这不只是因为强极性化合物的分离问题在各个领域引起了重视,而且因为与RPLC比较,HILIC具有流动相组成黏度低、色谱柱渗透性好、与质谱联用的灵敏度高及反压较低等优势。本文简要概述了HILIC的发展历程、特点及保留机理,重点介绍了HILIC用于环境分析的最新进展,评述了HILIC及RPLC用于污染物分析的优缺点,并指出了HILIC用于环境分析的未来发展趋势。
Hydrophilic interaction chromatography （HILIC） has been used as a supplement method of reversed-phase liquid chromatography （RPLC） for the separation of the polar and hydrophilic compounds, and has been received more and more attention in recent years. One reason is that the separation problems of strongly polar compounds are rising in various fields. Another is that there are some obvious advantages of HILIC compared with RPLC in the separation of polar compounds, such as low viscosity of mobile phase, good permeability of the column, high sensitivity when coupled to mass spectrometry and low back pressure. In this review, the development history, characteristics and retention mechanism of HILIC are summarized. The main attention in this review is on the applications of HILIC in environmental analysis. In addition, the advantages and disadvantages of HILIC and RPLC in the separation of pollutants are discussed, and the development trends of HILIC applications in environmental analy sis are proposed.
出处
《色谱》
CAS
CSCD
北大核心
2012年第3期232-238,共7页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(No.21177072)
中国科学院生态环境研究中心环境水质学国家重点实验室开放基金项目(No.2011-006)
国家水体污染控制与治理科技重大专项(No.2012ZXo7104-002-02)
关键词
亲水作用色谱
环境分析
应用
综述
hydrophilic interaction chromatography （HILIC）
environmental analysis
applica-tions
review
