摘要
建立了N-酰基高丝氨酸内酯类(N-acyl-homoserine lactones,AHLs)群体感应信号分子的气相色谱-质谱(GC-MS)检测方法,并优化了检测条件。在优化条件下,采用选择离子扫描模式(SIM)从细菌Pseud-oalteromonas sp.NJ6-3-1培养液中检测到3种AHLs(N-己基高丝氨酸内酯(C6-HSL)、N-辛基高丝氨酸内酯(C8-HSL)和N-十四基高丝氨酸内酯(C14-HSL))。与传统的薄层层析-生物传感器(TLC-biosensor)和高效液相色谱法(HPLC)相比,该方法更加简单、高效,只需对培养液样品进行简单处理,即可定性检测样品中的AHLs分子。该方法为从复杂基质中检测和鉴定AHLs信号分子提供了有效手段。
A rapid and sensitive method for the determination of N-acyl-homoserine lactones（ AHLs）, a kind of quorum sensing signal molecules in Gram-negative bacteria, was established based on gas chromatography - mass spectrometric technique. The GC - MS conditions for the detection of AHLs were optimized. Under the optimal conditions, three kinds of AHLs, e. g. N-hexanoyl-L-homoserine lactone （ C6 - HSL）, N-octanoyl-L-homoserine lactone （ Cs - HSL） and N-tetradecanoyl-L-homoserine lactone（C1, - HSL）, were detected in the cultured supernatants of bacteria Pseudoalteromonas sp. NJ6 -3 -1 under selected ion monitoring（SIM） mode. Compared with the traditional TLC-biosensor and HPLC method, this method is rapid and simple. AHLs in the samples could be detected qualita- tively only by a simple sample handling procedure. The study provides a useful method in the detec- tion of AHLs from complicated mixtures.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第3期347-350,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金面上项目(40776098)
河南大学科研基金项目(B2011037)
关键词
N-酰基高丝氨酸内酯
气相色谱-质谱法
群体感应信号分子
细菌
N-acyl-homoserine lactones
gas chromatography - mass spectrometry （ GC - MS）
quorum sensing signal molecule
bacteria
