摘要
环二鸟苷酸(cyclic diguanylate,c-di-GMP)是在细菌中普遍存在的第二信使分子,参与调节多种生理功能,包括细胞分化、生物被膜形成、致病因子产生等。细菌细胞内c-di-GMP合成与降解代谢分别受二鸟苷酸环化酶(diguanylate cyclase,DGC)和磷酸二酯酶(phosphodiesterase,PDE)调控,DGC和PDE共处于同一个蛋白中,是一个双功能蛋白酶的两个区域,分别负责菌体内c-di-GMP的合成和降解。c-di-GMP作用菌体内下游靶点包括PilZ结构域和GEMM核开关两种类型。目前发现c-di-GMP核开关是唯一不参与代谢活动而参与信号传导的一类核开关。本文综述了c-di-GMP的代谢途径、调控机制、生物学功能,以及c-di-GMP结构类似物合成及生物学评价等方面的最新研究进展。
Cyclic diguanylate （c-di-GMP） is a ubiquitous second messenger present in a wide variety of bacteria, which is responsible for cell differentiation, biofilm formation, pathogenic factor generation, and so on. The level of c-di-GMP in bacteria is regulated by two opposing active domains, diguanylate cyclase （DGC） and phosphodiesterase （PDE）, which are present in the same bifunctional protein, and in charge of the synthesis and the degradation of c-di-GMP, respectively. The target of c-di-GMP in the bacterial cell consists of PilZ domain and GEMM riboswitch, the only riboswitch that involved in signal transduction. of c-di-GMP, focusing on its metabolic pathway, regulatory mechanism, biological synthesis of c-di-GMP analogues and their biological activity. This article gives an overview function of c-di-GMP, and the synthesis of c-di-GMP analogues and their biological activity.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第3期307-312,258,共6页
Acta Pharmaceutica Sinica
