摘要
目的:研究慢性脑缺血大鼠海马CA1区锥体细胞树突形态及树突棘密度的变化。方法:对大鼠进行双侧颈总动脉永久性结扎(2VO)制备慢性脑缺血模型,分别于2周、4周、8周通过Morris水迷宫对各组大鼠进行行为学评价,筛选造模成功大鼠,进行Golgi染色,光镜下观察海马CA1区锥体细胞树突的分支、长度及树突棘密度的变化。结果:与对照组相比,4周、8周模型组树突的分支及长度显著减少(P<0.01),各周模型组树突棘的密度均有显著减少(P<0.01);模型组内随着缺血时间延长,树突的分支及长度、树突棘密度均显著减少(P<0.05)。结论:慢性脑缺血可导致海马CA1区锥体细胞树突及树突棘损伤性变化,从而构成进展性认知功能障碍的病理生理学基础。
AIM： To investigate the changes of dendritic morphology and spine density in hippocampal CA1 pyramidal cells of the chronic cerebral ischemic rats.METHODS： The model of chronic cerebral ischemia was established by permanent occlusion of the bilateral common carotid arteries（2VO） in rats.Two weeks,4 weeks or 8 weeks later,the behavior of the rats in each group was evaluated through the Morris water maze to select the successful modeling,and the brains were collected for processing Golgi staining.The changes in dendritic branch and length,and spine density in hippocampal CA1 pyramidal cells were observed under optical microscope.RESULTS： Compared with sham-operated group,dendritic branch and length in model group was significantly reduced in 4-week group and 8-week group（P0.01）,and spine density in model group were significantly reduced in 2-week,4-week and 8-week groups（P0.01）.With prolonged ischemia,dendritic branch and length,and spine density in model group were all significantly reduced（P0.05）.CONCLUSION： Chronic cerebral ischemia leads to traumatic changes in dendrites and spines in hippocampal CA1 pyramidal cells,which constitutes the pathophysiological basis in the progressive cognitive dysfunction.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第1期177-180,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81000530)
河南省卫生厅资助项目(No.200903028)
关键词
脑缺血
海马锥体细胞
树突
树突棘
Brain ischemia
Hippocampal pyramidal cells
Dendrite
Dendritic spine
