摘要
以多胺化壳聚糖为载体,戊二醛为交联剂固定化白毒鹅膏菌漆酶,研究了最佳固定化条件及固定化酶的部分性质.结果表明,戊二醛浓度2%,交联时间14 h,固定化时间6 h,给酶量70 mg/g载体时固定化效果最佳,酶活力可达64.3 U/g.此固定化漆酶的Km为8.73 mmol.L-1;最适pH值4.2,较自由漆酶向酸性偏移;最适反应温度为50℃,较游离漆酶热稳定性大大提高.
Laccase has been immobilized on polyethyleneaminated chitosan with glutaraldehyde as crosslinking agent.When glutaraldehyde concentration is 2%,linking time is 14 h,immobilization time is 6 h and laccase dosage is 70 mg/g carrier the optimal condition was obtained.In this way 64 U/g of immobilized enzyme was available.The Km for the immobilized enzyme is 8.73 mmol·L-1 and the optimal pH and temperature for immobilized enzyme are 4.2 and 50 ℃.Compared with the free enzyme,its stability is considerable improved.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第5期125-128,共4页
Journal of Henan Normal University(Natural Science Edition)
关键词
多胺化壳聚糖
固定化
漆酶
polyethyleneaminated chitosan
immobilization
fungal laccase
