摘要
目的检测不育男性患者精浆中免疫球蛋白IgA、IgG、IgM和精子活力与密度,分析各参数之间的相关性,探讨其与男性不育的关系。方法 96例不育症患者分为弱精子症组、少精子症组和无精子症3组,以31例精液质量正常的已生育男性作为参照对象。利用免疫比浊法和精子质量图文分析系统分别检测精浆中IgA、IgG、IgM和精子活力与密度。结果少精子症和无精子症患者精浆中IgM含量与正常组和弱精子症组比较显著减低(P<0.01,P<0.05);精浆中IgA、IgG和IgM之间两两相关(P<0.01),IgM含量与精子密度显著相关(P<0.05);不育症组与正常组比较,IgA、IgG和IgM相互之间的相关性减低。结论精浆中三种免疫球蛋白含量的失衡及IgM含量减少可能是导致男性不育的原因之一,该结果可为男性不育的研究提供参考。
Objective To investigate the impact and correlation between immunoglobin IgA,IgG and IgM in seminal plasma and sperm density and activity in infertile males.Methods Semen samples of 96 cases of infertile males,divided into three groups including normozoospermia,oligozoospermia and azoospermia group,and 31 fertile males（control group） were collected and detected for IgA,IgG and IgM in seminal plasma by immunology turbidimetric method and for sperm density and activity by CACS according to World Health Organization guidelines.Results The concentration of IgM in seminal plasma of oligozoospermia group and azoospermia group were significantly lower than those of control group and normozoospermia group （P0.01,P0.05）.Correlations were demonstrated between IgA,IgG and IgM in seminal plasma（P0.01）.IgM level and sperm count were correlated （P0.05）.Compared to control group,the correlation of IgA,IgG and IgM in seminal plasma were decreased in infertile groups.Conclusion The level imbalance of immunoglobulin and the decreased concentration of IgM in seminal plasma might play an essential role in infertility of males,providing reference for related research of male infertility.
出处
《国际检验医学杂志》
CAS
2012年第2期166-167,共2页
International Journal of Laboratory Medicine
