摘要
目的通过对比研究了解微创食管癌手术与传统开放术式术后C反应蛋白(C-reactive protein,CRP)及细胞因子水平变化。方法选择2009年1月至2011年2月在重庆医科大学第一附属医院胸外科接受微创食管癌切除术(minimally invasive esophagectomy,MIE)与开胸食管癌切除术(open esophageal resection,OPEN)的患者各10例,分别于术前,术后4、24、48、72、120、168 h抽取静脉血标本,用双抗体夹心ABC-ELISA法测定TNF-α、IL-6,用超敏CRP分析测定CRP值。结果 2组人群术前、术后血浆TNF-α水平差异没有统计学意义,2组术后4、24、120 h血清CPR水平差异有统计学意义(P<0.05),2组术后各时间点血清IL-6水平差异均有统计学意义(P<0.05);两因素重复测量资料的方差分析提示MIE组和OPEN组术后不同时间点血清IL-6细胞因子浓度差异有统计学意义(P<0.01),且手术方式与时间因素之间存在交互作用。结论微创食管癌手术与传统食管癌手术相比,可能会减少术后机体炎症因子释放,减少术后炎症反应。
Objective To investigate different responses of preoperative and postoperative serum levels of C-reactive protein（CRP）,tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in minimally invasive esophagectomy（MIE） and traditional open esophageal resection（OPEN）.Methods Twenty patients receiving MIE（MIE group） and traditional OPEN（OPEN group） in thoracic surgery department of the First Affiliated Hospital of Chongqing Medical University from Jan 2009 to Feb 2011 were chosen（n=10）.High-sensitive CRP analysis and double-antibody sandwich ELISA（ABC-ELISA） were applied to detect CRP value and serum levels of TNF-α and IL-6 before and at 4,24,48,74,120 and 168 h after operation,respectively.Results The differences of preoperative and postoperative serum levels of TNF-α of the two groups were not statistically significant（P0.05）,while the differences of serum levels of CRP at postoperative 4,24 and 120 h were statistically significant（P0.05）,as well as IL-6 level at each postoperative time point（P0.05）.Two-factor repeatedly measured analysis of variance indicated that there was statistically significant difference of IL-6 level at each postoperative time point between the MIE and OPEN groups,and there was an interaction between operation type and time.Conclusion Compared with traditional OPEN,MIE can possibly reduce postoperative release of inflammatory cytokines and inflammatory responses.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第3期268-270,共3页
Journal of Third Military Medical University
关键词
微创手术
食管癌
细胞因子
C反应蛋白
minimally invasive surgery
esophageal cancer
cytokine
C-reactive protein
