摘要
目的研究强直性脊柱炎(AS)中环境因素与FcRL基因的交互作用。方法对169例AS患者环境因素进行调查,PCR-SSP技术检测HLA-B27亚型,连接酶检测反应方法检测FcRL3、FcRL5基因SNP位点,采用单纯病例研究方法就AS基因与环境交互作用进行研究。结果本次研究中169例AS病人HLA-B27共检测出B*2704、B*2705、B*2710、B*2702和B*2715五个亚型;FcRL3基因中rs7522061位点与饮食偏咸存在交互作用,与其他环境因素无交互作用;FcRL5基因中rs12036228位点只与饮食偏咸因素存在交互作用;FcRL5基因rs6427384位点分别与饮酒和饮食偏咸有交互作用。结论饮食偏咸可能作为环境风险因素与易感基因FcRL5共同作用增加AS的发病风险;适度饮酒可能减低FcRL5基因(rs6427384)诱发AS的危险性;FcRL3基因(rs7522061)在存在饮食偏咸因素时起到效应修饰作用,增加疾病风险。
Objective To study interaction of FcRL gene and environmental factors in ankylosing spondylitis.Methods 169 AS patients were genotyped using a panel of single-nucleotide polymorphism （SNP） markers within FcRL3 and FcRL5 by lipase detection reactions （LDR）.Polymerase chain reaction （PCR） using sequence-specific primer （SSP） methods were used to determine HLA-B27 subtypes.We analyzed the interaction of G×E in AS patients by using a case-only study.Results From 169 patients with AS,five HLA-B27 subtypes were detected in our study,including B＊2704,B＊2705,B＊2710,B＊2702,B＊2715;Salty taste interacts with FcRL3 rs7522061 and FcRL5 rs12036228;there are interactions of FcRL5 rs6427384 with drinking and salty taste.Conclusion Salty taste may be a risk exposure factor to interact with FcRL5 gene which may increase the incident risk of AS.Moderate alcohol intake may reduce the susceptibility of AS causing by FcRL5 rs6427384.FcRL3 rs7522061 would take an modification effect with salty taste in AS patients.
出处
《中国卫生统计》
CSCD
北大核心
2011年第6期617-619,622,共4页
Chinese Journal of Health Statistics
基金
国家自然科学基金(编号:30771849,30972530)
安徽省自然科学基金(编号:090413133)
2009年安徽省学术和技术带头人后备人选计划
