摘要
目的:建立雪山金罗汉止痛涂膜剂中延胡索乙素的含量测定方法。方法:采用HPLC法对制剂中主要成分延胡索乙素进行定量分析。CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸溶液(用三乙胺调节pH至6.0)(38∶62),检测波长280 nm,柱温30℃,流速1.0 mL.min-1。结果:延胡索乙素在0.010 84～0.130 08μg呈良好的线性关系,平均回收率为97.74%,RSD 1.75%(n=9)。结论:方法准确可靠,专属性强,结果稳定,重复性好,可有效控制制剂质量。
Objective：To establish an method for determination of tetrahydropalmatine in Xueshan Jinluohan Zhitong Balm.Method： The tetrahydropalmatine in Xueshan Jinluohan Zhitong Balm was determined by HPLC.CAPCELL PAK C18（4.6 mm×250 mm 5 μm）column was used.The modbile phase was acetonitrile-0.1% phosphoric acid（adjusted pH to 6.0 with triethyplamine）（38∶62） with the flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and UV detection wavelength was at 280 nm.Result：The tetrahydropalmatine were linear at the range of 0.010 84-0.130 08 μg,respectively.The average recovery was 97.74% and RSD was 1.75%（n=9）.Conclusion：The established method is accurate,reliable and specific.The quality of Xueshan Jinluohan Zhitong Balm can be controlled by the method.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期106-108,共3页
Chinese Journal of Experimental Traditional Medical Formulae
