摘要
有毒物质Hormesis效应对传统的毒理学模型提出了挑战。在低剂量条件下单纯的效应增强并不一定是Hormesis效应,可能由效应的某种波动或试验误差引起。对有毒物质Hormesis效应特征、发生的低剂量范围及其识别与评价方法进行了综述,并以低剂量镉对蚯蚓体内谷胱甘肽过氧化物酶(CSH-PX)活性影响为例,建立了Hormesis效应及其定量评价方法。结果表明,二次函数拟合模型为Y=0.3994-1.4472X^2+1.707X,刺激效应强度为45.34%。
The present paper takes as its objective to provide ways to identify and evaluate the hormesis induced by toxic substances. Great challenges have been brought about to the traditional model of toxicology, and the general stimulation at low-dose began to attract more and more attention both at home and abroad. However, the current research advances in this aspect are mostly based on the accumulated observation results compared to the control at low-dosages. Nevertheless, merely enhanced responses at low doses may not be likely to reveal fully the characteristics of hormesis but a random fluctuation in the response process. Thus, it can be said that the key problem leading to the inefficient identification and evaluation of hormesis lies in the lack of identification and evaluation means. Therefore, in this paper, we have first of all summarized the characteristic features of hormesis, the relation of low-dose range and the evaluation methods, and, what is more, worked out a new approach to the development of hormesis. To illustrate how to use the method, we have made attempt to apply it to the data analysis due to the influence of the low dose cadmium on the glutathione peroxidase （GSH-PX） activity in the earthworms. The results of our experiments show that cadmium at low concentrations tends to induce the stimulation of GSH-PX activity, whereas high concentrations tend to result in inhibition. To make the data fit the quadratic functions, let the function be expressed as Y=0.3994 1.4472X2＋1.707X, which can be reflected in an inverted U-shape curve. The area under the hormetic zone and the magnitude of the stimulatory response could be obtained. The adoption of the direct integral method may help us to work out the hormetic area with AUCH and AUCZEP while the ratio of AUCH and AUCZEP is 45.34%. When we conduct a dose-response experiment and enhance responses at some low concentrations, statistical analyses should be done to determine if the enhanced responses result in chance occurrence. Therefore, we have pointed out in this paper that the incidence of hormesis can be statistically evaluated by using the ratio of AUCH and AUCZEP, which implies that the stated method proposed in this paper is mainly applicable to the continuous data.
出处
《安全与环境学报》
CAS
CSCD
北大核心
2011年第6期24-27,共4页
Journal of Safety and Environment
基金
国家自然科学基金项目(20877054)
关键词
环境科学技术基础学科
HORMESIS
有毒物质
模型拟合
评价方法
basic disciplines of environmental science and technology
normesis
toxic substance
model fitting
estimating method
