摘要
目的建立高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,测定并比较天然牛黄与酶促牛黄中胆固醇的含量。方法色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm);以乙腈-异丙醇(1∶1)为流动相,流速1.0 mL.min-1;漂移管温度67.5℃,气体流速1.7 L.min-1。结果胆固醇浓度在4.0～20.0μg.mL-1范围,浓度对数(logC)与峰面积对数(logA)呈良好的线性关系logA=1.541 2logC-2.525(r=0.999 2);低、中、高浓度平均回收率分别为98.41%,100.04%,96.46%,RSD分别为0.63%,1.67%,1.03%。结论该法简便、准确,专属强,可用于牛黄、牛黄替代品及其制剂的质量控制。
Objective To establish a HPLC-ELSD method for determining cholesterol and making a comparation of which between two kinds of calculus bovis. Methods A C18（250 mm×4.6 mm,5 μm）column was used with a mobile phase as acetonitrile-Isopropanol（1：1）flowing at 1.0 mL·min-1.The temperature of drift tube was 67.5 ℃,and the gas flow rate was 1.7 L·min-1. Results The cholesterol was in a good linear relationship in the range of 4.0-20.0 μg·mL-1,logA=1.541 2logC-2.525（r=0.999 2）.The average recovery was 98.41%,100.04%,96.46%,respectively,and RSD was 0.63%,1.67%,1.03%,respectively. Conclusion The method is simple,accurate,specific and can be used for the quality control of cholesterol in natural calculus bovis,succedaneums and preparations.
出处
《医药导报》
CAS
2011年第12期1633-1636,共4页
Herald of Medicine
基金
山东省教育厅基金资助项目(基金编号:J10LF16)
