摘要
采用0(CK)、50、100、150、200和250mmol/L 6个NaCl浓度梯度,对6份虉草(Phalaris arundinacea)材料的种子萌发和幼苗生长2个阶段进行盐胁迫处理。通过对种子的发芽率、鲜质量、苗高、根长、脯氨酸含量、丙二醛和叶绿素含量等生理化指标的测定,观测其在盐胁迫下的一系列生理生化反应。结果表明,低NaCl浓度对种子萌发及幼苗生长这2个阶段的影响较小,随着NaCl浓度的增高,种子发芽率、根长、鲜质量、苗长、叶绿素含量逐渐降低,脯氨酸含量显著增加,丙二醛含量呈先降低后增加的趋势。6份虉草材料在种子发芽和幼苗生理变化上均表现出不同的耐盐能力,Zxy06p-2449、Zxy06p-1904耐盐能力较强,其次为Zxy06p-2380、Zxy06p-2633,Zxy06p-2262和Zxy06p-1916的耐盐性较差。
An experiment was conducted to determine the effect of NaCl stress on seed germination and physiological and biochemical characteristics of seedlings of 6 accessions Phalaris arundinacea by measuring seed germination rate,fresh weight,shoot length,root length,proline content,chlorophyll and malonaldehyde（MDA） content.The NaCl stress consisted of 0（CK）,50,100,150,200 and 250 mmol/L.The results of this study showed that lower NaCl concentration did not significantly affected seed germination and seedling growth of P.arundinacea;however,the germination rate,fresh weight,shoot length,root length and chlorophyll content of P.arundinacea decreased,and the proline content significantly increased,and MDA content decreased firstly decreased and then increased as the NaCl concentration increased.This study indicated that 6 accessions of P.arundinacea had the different salt tolerance in seed germination and physiological property of seedling,in which Zxy06p-2449 and Zxy06p-1904 were the strongest salt tolerant,and Zxy06p-2380 and Zxy06p-2633 were intermediate,and Zxy06p-2262 and Zxy06p-1916 were sensitive to salt stress.
出处
《草业科学》
CAS
CSCD
北大核心
2011年第12期2136-2142,共7页
Pratacultural Science
基金
科技支撑2009BADA7B04
"973"课题(2012CB215300)
国家现代牧草产业技术体系(CARS-35)
北京市重点实验室
