摘要
目的：探讨建立高效液相色谱法测定青藤碱微乳中青藤碱含量的方法。方法：采用ODS-C18色谱柱,以乙腈-0.01%磷酸二氢钾溶液（用三乙胺调pH至7.8）（40∶60）为流动相,检测波长为262 nm,流速1.0 mL/min,柱温为室温,进样量为20μL。结果：青藤碱在40.6~203μg/mL范围内线性关系良好,线性回归方程：A=9 934.5C＋9 839.8（r=0.9999）,平均回收率为102.36%（RSD=4.02%）。结论：高效液相色谱法简便、快速、灵敏、重复性好,可作为青藤碱微乳含量测定方法。
Objective： To establish a HPLC method for the determination of sinomenine microemulsion.Methods： An intersil ODS-C18 column was used with the mobile phase acetonitrile-0.1% potassium dihydrogen phosphate（pH7.8 adjusted by triethylamine）（40：60） at a detection wavelength 262 in an injection volume 20μl;The flow rate was 1.0ml/min and column temperature was room temperature;Results： sinomenine had good linear relation in the range of 40.6-203μg/ml.The average recovery was 102.36%（RSD=4.02%）.Conclusion： HPLC is simple,fast,sensitive and fit for the concentration control of the dispersible tablets.
出处
《交通医学》
2011年第5期453-455,共3页
Medical Journal of Communications
关键词
青藤碱
高效液相色谱法测定
微乳
Sinomenine
High performance liquid chromatography Microemulsion
Content
