摘要
实验材料系大连海洋大学农业部海洋水产增养殖学重点开放实验室培育的虾夷扇贝"象牙白"家系和普通褐色虾夷扇贝家系,并与大连獐子岛海区养成的F2代9月龄个体各60枚。用22个具较高多态性的虾夷扇贝(Patinopecten yessoensis)微卫星引物对其进行SSR扩增并分析。结果显示,不同多态位点的期望杂合度(He)、等位基因数(Na)、有效等位基因数(Ne)、PIC平均值等指标表明普通褐、白贝的遗传多样性水平较高且没有显著差异。实验中发现座位8在普通褐贝大部分个体中能获得特异条带A,但在白贝所有个体中均未见,推断特异条带A为白贝特异阴性条带。对22个微卫星标记与虾夷扇贝生长性状相关性进行了方差分析和多重比较,结果表明,座位HLJX109、HLJX178和HLJX139与体重呈显著(P<0.05)或极显著(P<0.01)相关,座位8、HLJX191和HLJX139与壳长和壳高呈显著(P<0.05)或极显著(P<0.01)相关。笔者分析了微卫星分子标记与虾夷扇贝壳色和生长性状的相关性,为虾夷扇贝的相关性状进一步QTL定位提供参考数据,并为虾夷扇贝的养殖和选育提供理论指导。
The ordinary Patinopecten yessoensis （control groups） and ＂Ivory＂ strains of Patinopecten yessoensis F 2 cultured in Dalian Ocean University,Key Laboratory of Mariculture,Ministry of Agriculture were cultured to adults in Zhangzidao sea （9-month-old,60 samples respectively） and used in this experiment,the genetic structure of the two groups were analysed using 22 polymorphic microsatellites markers.The value of H e,N a,N e and PIC showed that the genetic diversity of the two groups were high and there was no significant difference in genetic structure between the two groups.The band A at locus 8 appeared in most individuals of brown scallops,but it disappeared completely with no amplification in ＂Ivory＂ scallops;it was suggested that this band A was possibly the negative genetic marker in white scallops.The SPSS 16.0 was used to analyze the effect of these 23 microsatellites on growth traits.Results indicated that locus HLJX 109,HLJX 178 and HLJX 139 had a significant impact （P0.05 or P0.01） on body weight;locus 8,HLJX 191 and HLJX 139 had a significant impact （P0.05 or P0.01） on shell length and shell height.This search was working on the relationship between Patinopecten yessoensis＇s microsatellites markers and with different shell colours and growth traits in order to provide data for further study on Patinopecten yessoensis＇s QTL locations and provide theoretical guidance for Patinopecten yessoensis＇s cultivation and breeding.
出处
《中国农学通报》
CSCD
北大核心
2011年第26期83-91,共9页
Chinese Agricultural Science Bulletin
基金
国家"863"重大项目资助"扇贝高产
抗逆品种培育"(2006AA10A408)
农业部引进国际先进农业科学技术计划(2006-G5515)
辽宁省重大项目"渔业新品种引进
开发"(2008203001)
国家自然科学基金资助项目"虾夷扇贝生长性状QTL定位研究"(30671594)
关键词
虾夷扇贝
壳色
微卫星
生长性状
相关分析
Patinopecten yessoensis
shell colours
SSR
growth traits
correlation analysis
