摘要
目的:揭示微波解冻对冻结组织免疫组化染色效果的影响,为用微波解冻冷冻组织进行免疫组化染色提供实验依据。方法:将清洁级大耳白家兔分为常规对照组、自然解冻组、温水解冻组、微波解冻组。用空气将家兔栓塞致死后取其肝、肺、肾组织,包埋并制成切片,进行广谱细胞角蛋白免疫组化染色并观察染色结果。结果:微波解冻对肝、肾、肺冻结组织中细胞角蛋白抗原的影响不明显,均能正常显色。结论:冻结组织微波解冻对细胞角蛋白的免疫组化检查未见明显不良影响,提示温和的微波解冻对组织中细胞角蛋白抗原影响不明显。
Objective： Using immunohistochemistry methods to detect the affection of microwave thawing to frozen tissue on CK.Methods ： The clean-level big-ear white rabbits were divided into 4 group,normal,natural thawing,warm water thawing and microwave thawing group.Rabbits were killed by air embolism.After which the liver,lung and kidney tissue sections were embedded,sliced,HE stained,and immunohistochemical stained with cytokeratin（CK）.Results： The affection of microwave thawing to frozen tissue on CK antigen was not obvious among the different groups.Liver,kidney and lung tissue showed normal staining respectively.Conclusions： Moderate microwave thawing to frozen tissue on CK stained by immunohistochemistry has no significant adverse affection.
出处
《中国医学物理学杂志》
CSCD
2011年第5期2941-2944,共4页
Chinese Journal of Medical Physics
