摘要
Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.
Five hybridomas stably secreting monoclonal antibodies （McAbs） to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin （BSA） as antigen. These McAbs showed slightly high cross-reactivity with testosterone （8.1—12.3%） and estrone （0.8—2.5%） and high affinities ranging from 2.0×10<sup>7</sup> to 2.8×10<sup>8</sup> L/M. They were all of the IgG<sub>1</sub> subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone （P） and luteinizing hormone （LH） than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.
基金
Project supported by one of the National Research Subjects in the Seventh Five-Year Science and Technology Plan and the National Natural Science Foundation of China
