摘要
目的:以秋水仙碱为对照,观察冬虫夏草对实验性肝纤维化的治疗效果,并探讨其机理。方法:用CCl_4诱导大鼠肝纤维化,分别以生理盐水、秋水仙碱、冬虫夏草各24只进行治疗。实验第4,8,12,14周分批处死动物,测量血清Ⅲ型前胶原含量,取肝组织进行病理观察,并用免疫组化技术观察肝组织Ⅰ,Ⅲ,Ⅳ型胶原,结蛋白阳性细胞的动态变化。结果:两种药物均能明显降低血清Ⅲ型前胶原含量,并减轻肝组织Ⅰ,Ⅲ,Ⅳ型胶原的沉积,最终防止肝假小叶及肝硬化形成。动物经药物治疗后肝组织内结蛋白阳性细胞数均明显低于生理盐水组。结论:冬虫夏草与秋水仙碱均有良好的抗肝纤维化作用,它们能抑制肝内储脂细胞(结蛋白阳性细胞)的增殖和转化,从而减少胶原合成及在肝窦周沉积。冬虫夏草无毒副作用,可能是一种有前途的抗肝纤维化药物。
AIM To study on the effects of cordyceps sinensis in liver fibrosis,and its mechanism.Colchicine was used as control therapy.METHODS Liver fibrosis was induced by CCl4 in Wistar rats.The animals were treated with cordyceps sinensis)n=24),colchicine)n=24),and normal saline)n=24),respectively.The rats were killed in batches at 4.8.12.14 weeks.Serum procollagen Ⅲ(PCⅢ)was measured by radioimmoassay and liver tissue was observed pathologically.Dynamic changes of type I,Ⅲ,Ⅳ collagens and desmin-positive cells in liver tissue were studied by immunohistochemical technique.RESULTS Cordyceps sinensis and colchicine were both able to markedly reduce serum PCⅢ level,and reduce Ⅰ,Ⅲ,Ⅳ collagen deposition in liver tissue,and then prevent the formation of pseudo-lobule and cirrhosis.Desmin-positive cell number in liver tissue in two treated groups was much lower than that in normal saline one.CONCLUSION Both Cordyceps sinensis and colchicine had satisfactory effect on hepatic fibrosis.They can inhibit proliferation and transition of hepatic fat-storing cells)desmin-positive cells),so as to reduce collagen synthesis and deposition in liver.Cordyceps sinensis may be a useful anti-fibrotic drug in chronic liver diseases as it has no side-effect.
出处
《世界华人消化杂志》
CAS
1994年第4期208-209,196,共3页
World Chinese Journal of Digestology
关键词
冬虫夏草
秋水仙碱
肝纤维化
大鼠
cordyceps sincnsis colchicine rat liver fibrosis
