摘要
目的:观察益肾颗粒对糖尿病肾病大鼠肾脏的保护作用。方法:采用单侧肾切除加链脲佐菌素55 mg.kg-1一次性ip复制糖尿病肾病大鼠模型,试验分单切肾组,模型组,益肾颗粒低、高剂量组(8.1,16.2 g.kg-1),阳性对照药洛汀新组(1.5×10-3 g.kg-1)。ig,1次/d,连续16周,单切肾组和模型组给予等容积蒸馏水,观察药物对大鼠血清层黏连蛋白、Ⅳ型胶原含量以及肾组织超微结构的影响。结果:益肾颗粒高剂量组能明显降低大鼠血清层黏连蛋白和Ⅳ型胶原含量(P<0.05),高、低剂量组能减轻肾小球滤过膜足细胞足突融合、基底膜的增厚以及毛细血管内皮细胞增生。结论:益肾颗粒是通过降低血清层黏连蛋白和Ⅳ型胶原含量,减轻肾小球超微结构病理性变化,从而起到保护肾脏的作用。
Objective： To observe the protective effects of Yishen granule on renal lesion in diabetic nephropathy（DN） rats.Method：DN rats with unilateral nephrectomy induced by STZ with single intraperitoneal injection at the dose of 55 mg·kg-1.Then the rats were randomly divide into the unilateral nephrectomy group,DN model group,Yishen granule groups in low and high dose（8.1,16.2 g·kg-1）,the positive control medicine group with lotensin（1.5×10-3 g·kg-1）.The different drugs were given by intragastrical administration once a day and lasted 16 weeks.The rats in the unilateral nephrectomy group and DN model group were given isovolumetric distilled water.It was been observed that the effects of the different drugs on the serum levels of laminin（LN） and type Ⅳ collagen,as well as renal ultramicrostructure.Result： The serum levels of LN and type Ⅳ collagen were significantly decreased in Yishen granule high-dose group.It was been observed that the foot processes of podocytes,basement membrane thickening and endocapillary proliferation in glomerular filtration membrane were significantly alleviated in two Yishen granule groups.Conclusion： Yishen granule could improve renal tissue by decreasing the serum levels of LN and type Ⅳ collagen and inhibiting pathological changes of glomerular ultramicrostructure in DN.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第22期213-215,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项综合性中药新药研究开发技术大平台(2009ZX09301-005)
国家科技重大专项重大新药创制(2009ZX09303-003)
