摘要
目的:研究玄参破壁粉粒的体内抗菌作用。方法:金黄色葡萄球菌和绿脓杆菌分别培养18 h增殖后,用5%酵母浸出粉溶液稀释成不同浓度,ip感染小鼠,选用80%～100%致死率的金黄色葡萄球菌和绿脓杆菌的稀释液,感染给药小鼠,观测给药小鼠在染菌后7 d内的平均存活天数和死亡率。结果:玄参破壁粉粒3.5,2.8,2.1,1.4 g.kg-1及其常规饮片的3.5 g.kg-1剂量均能不同程度的抑制金黄色葡萄球菌小鼠的死亡率并延长平均存活天数;玄参破壁粉粒3.5,2.8 g.kg-1及其常规饮片的3.5 g.kg-1剂量均能不同程度的抑制绿脓杆菌小鼠的死亡率并延长平均存活天数。结论:玄参破壁粉粒直接给药具有一定的体内抗菌作用,与常规饮片的提取液体内抗菌作用等效。
Objective： To study the antibacterial effect of Scrophulariae Radix particle in infected mice.Method： Staphylococcus aureus and Pseudomonas aeruginosa were cultured for 18 hours and then 5% yeast liquor was added each of them to a suitable level which made 80%-100% infected mice death.Then each of the suitable bacterial liquor was inoculated into the abdominal cavity of mice who had given corresponding medicines for 7 days.Death rate and average survival time were both observed and analyzed within 7 days after infected.Result： 3.5,2.8,2.1,1.4 g·kg-1 of Scrophulariae Radix particle and 3.5 g·kg-1 dose of conventional processed product can inhibit the death rate caused by S.aureus in mice and extend the average survival time;3.5,2.8 g·kg-1 Scrophulariae Radix particle and 3.5 g·kg-1 dose of conventional processed product can inhibit the death rate caused by P.aeruginosa in mice and prolong the average survival days.Conclusion： Directly administrated Scrophulariae Radix particle has the equivalent antibacterial activity with conventional processed products.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第22期178-180,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2008年度粤港关键领域重点突破招标项目(200801)
关键词
玄参
破壁粉粒
金黄色葡萄球菌
绿脓杆菌
Scrophulariae Radix
particle
Staphylococcus aureus
Pseudomonas aeruginosa
