摘要
目的研究Survivin基因沉默对人胃癌MGC-803细胞增殖和对化疗药物塞来昔布敏感性的影响。方法设计合成Survivin的siRNA序列,LipofectamineTM2000转染入MGC-803细胞。采用RT-PCR和Western blotting检测Survivin在干扰后mRNA和蛋白的表达情况,利用流式细胞仪检测细胞周期。通过MTT法和细胞克隆形成试验法察Survivin基因沉默后MGC-803细胞对塞来昔布的敏感性。结果 Survivin基因沉默48 h后,MGC-803细胞的Survivin基因和蛋白表达明显降低(P<0.05)。细胞周期被阻滞在G0/G1期,S期细胞数减少(P<0.05)。Survivin基因沉默组细胞对塞来昔布的敏感性显著性增强(P<0.05)。结论 Survivin特异性siRNA能显著沉默MGC-803细胞Survivin基因,抑制细胞增殖,并增强MGC-803细胞对塞来昔布的敏感性。
Objective To investigate the effect of small interfering RNA（siRNA）-mediated survivin knock-down on the proliferation of human gastric cancer MGC-803 cells and their sensitivity to celecoxib.Methods The siRNA against survivin was constructed and transfected into MGC-803 cells via LipofectamineTM 2000.The expression of survivin in the transfected cells was detected by RT-PCR and Western blot,and flow cytometry was used to detect the cell cycle changes.The sensitivity of the cells to celecoxib after transfection was examined using MTT assay and clonogenic assay.Results The protein and mRNA levels of survivin in MGC-803 cells were decreased significantly after siRNA transfection,which also caused cell cycle arrest in G0/G1 phase.The sensitivity of MGC-803 cells to celecoxib was significantly increased after siRNA transfection.Conclusion siRNA-mediated survivin silencing causes growth suppression of MGC-803 cells and enhances their sensitivity to celecoxib in vitro.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第11期1944-1948,共5页
Journal of Southern Medical University
