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一种新颖的油酸诱导乳猪急性肺损伤动物模型 被引量：7

A stable and reproducible piglet model of acute lung injury induced by injecting low-dose oleic acid
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摘要 目的采用间断小剂量油酸注射方法,构建符合肺损伤标准、血流动力学稳定的乳猪急性肺损伤动物模型。方法中华小型猪6只,经右心耳插管注射油酸-乙醇溶液0.1mL/kg,0.1mL/次,间隔90s直至注射完毕。注射过程中监测循环、呼吸参数;实验结束取右下肺组织标本行组织病理检查。结果油酸注射前、后心率、平均动脉压、平均肺动脉压、心输出量、动脉血氧分压、动脉血二氧化碳分压、pH值、氧合指数差异均有统计学意义(P<0.05)。组织病理显示双肺呈弥漫性改变。结论小剂量间断注射油酸可构建血流动力学稳定并符合肺损伤诊断标准的急性肺损伤动物模型。 Objective To develop a hemodynamically stable and reproducible piglet model of acute lung injury by acid-alcohol mi 0.1 mLandth injecting low-dose oleic acid. xture （0.1 mL/kg） via right Methods Six Chinese mini-piglets were injected with oleic atrial appendage cannula. The dose of each injection was e interval was 90 seconds. Arterial oxygen pressure and the fraction of inspired oxygen were dynamically monitored. Circulation and respiratory function data were monitored. Right lower lung was histopathologically detected. Results There were significant differences in heart rate, mean arterial pressure, mean pulmonary arterial pressure, cardiac output, arterial oxygen pressure, arterial partial pressure of carbon dioxide, pH value, and arterial blood gas and oxygenation index between pre- and post-injection of oleic acid（P〈0. 05）. The histopathologic examination showed diffuse lung changes. Conclusion A stable and reproducible piglet model of acute lung injury can be achieved by injecting low-dose oleic acid.
作者 朱耀斌 李晓锋 王强 张燕搏 范祥明 李志强 许耀强 凌峰 刘爱军 刘迎龙
机构地区 首都医科大学附属北京安贞医院小儿心脏中心 北京协和医学院中国医学科学院阜外心血管病医院小儿心脏中心
出处 《中华实用诊断与治疗杂志》 2011年第11期1088-1090,1093,共4页 Journal of Chinese Practical Diagnosis and Therapy
基金 国家自然科学基金(30471721) 国家自然科学基金(30670928) 国家自然科学基金(81070055) 科研基地-科技创新平台-提高重症复杂先心病患儿疗效的基础与临床研究(PXM2011_014226_07_000060) 北京市自然科学基金(7112046) 北京市医药产品和技术重大项目培育研究(Z101107050210020) 北京市科委"十二五储备项目"(Z11110006150000)
关键词 呼吸窘迫 肺损伤 油酸 乳猪 动物模型 Respiratory distress lung injury oleic acid immature piglets animal model
分类号 R725.6 [医药卫生—儿科] R-332 [医药卫生]
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参考文献13

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二级参考文献47

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共引文献30

同被引文献32

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引证文献7

二级引证文献46

中华实用诊断与治疗杂志
2011年 第11期

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