摘要
目的:考察柴胡总皂苷提取物的溶血作用和抗氧化剂对柴胡总皂苷溶血的影响。方法:通过肉眼观察和分光光度法考察不同浓度柴胡皂苷的溶血作用及抗氧化剂对柴胡总皂苷溶血的影响。结果:柴胡总皂苷质量浓度为在0.01,0.02 g.L-1时,其溶血曲线呈S形,溶血当量为0.01 mg,溶血指数为1∶10万,抗氧化剂与柴胡总皂苷组溶血率低于同浓度柴胡总皂苷组。结论:柴胡总皂苷具有一定的溶血作用,且溶血强度与浓度呈剂量依赖性,抗氧化剂的加入能减轻柴胡总皂苷的溶血作用。
Objective： To study hemolytic effect of extraction of saikosaponins and antioxidants on the hemolytic effect of extraction of saikosaponins.Method： The hemolytic effects by different concentrations of saikosaponins and antioxidant effects of saikosaponins hemolytic were observed by naked eye and UV.Result： For saikosaponins with concentrations of 0.01,0.02 g·L-1,its hemolytic curve showed s-shaped,hemolytic equivalent was 0.01 mg,hemolytic index was 1∶100 000,antioxidant and saikosaponins group hemolytic rate was lower than the concentration of saikosaponins group.Conclusion： Saikosaponins has hemolytic effect with a certain degree,and hemolytic strength and concentration is dose-dependent,addition of antioxidants can reduce the hemolytic effect of saikosaponins.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第20期166-169,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重大新药创制专项(2009ZX09103)
