摘要
以小麦品系pm35为材料,利用基因枪法将核酸酶barnase基因导入小麦,获得12株阳性苗。至开花期,有7株表现为花粉明显减少,经花粉染色及花粉萌发率测定显示,该7株转基因小麦存在不同程度的花粉败育现象。进一步计算结实率发现,其结实率都明显下降,其中转基因植株10-11的结实率仅为3.43%。
Barnase gene was transferred into a wheat line ＂pm35＂ by gene gun bombardment and 12 positive seedings were obtained.At flowering stage,the fertility of pollens of 7 plants reduced significantly through pollen staining and pollen germination test.The seed-setting rate decreased obviously,with the least of 3.43%（the 10-11 line）.
出处
《作物杂志》
CAS
CSCD
北大核心
2011年第5期27-30,共4页
Crops
基金
黑龙江省高校重点实验室开放基金项目(GS2009004)
东北农业大学创新团队课题(CX7002-4-2)
关键词
小麦
核酸酶基因
花粉活力
结实率
雄性不育
Wheat
Barnase gene
Pollen vigor
Seed-setting rate
Male sterility
